Citrus Vascular Proteomics Highlights the Role of Peroxidases and Serine Proteases during Huanglongbing Disease Progression
| Autor: | Shree Prasad Thapa, Fatta B. Gurung, Zhiqian Pang, Gitta Coaker, Veronica Ancona, Jessica Y. Franco, Nian Wang, Danielle M. Stevens, Thomas W. H. Liebrand |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
Citrus Proteome medicine.medical_treatment Messenger Orange (colour) Biochemistry Analytical Chemistry Gene Expression Regulation Plant bacteria Candidatus Liberibacter asiaticus Phylogeny Plant Proteins plant biology 0303 health sciences biology 030302 biochemistry & molecular biology food and beverages Peroxidases click chemistry Disease Progression Citrus × sinensis Peroxidase Biochemistry & Molecular Biology Proteases Quantitative proteomics huanglongbing Microbiology 03 medical and health sciences medicine Protease Inhibitors RNA Messenger Molecular Biology Plant Diseases 030304 developmental biology Protease Research Affinity proteomics Plant Gene Ontology Gene Expression Regulation biology.protein RNA proteases Phloem Serine Proteases Plant Vascular Bundle |
| Zdroj: | Molecular & cellular proteomics : MCP, vol 19, iss 12 Mol Cell Proteomics |
| ISSN: | 1535-9476 |
| DOI: | 10.1074/mcp.ra120.002075 |
| Popis: | Huanglongbing (HLB) is the most devastating and widespread citrus disease. All commercial citrus varieties are susceptible to the HLB-associated bacterium, Candidatus Liberibacter asiaticus (CLas), which resides in the phloem. The phloem is part of the plant vascular system and is involved in sugar transport. To investigate the plant response to CLas, we enriched for proteins surrounding the phloem in an HLB susceptible sweet orange variety, Washington navel (Citrus sinensis (L) Osbeck). Quantitative proteomics revealed global changes in the citrus proteome after CLas inoculation. Plant metabolism and translation were suppressed, while defense-related proteins such as peroxidases, proteases and protease inhibitors were induced in the vasculature. Transcript accumulation and enzymatic activity of plant peroxidases in CLas infected sweet orange varieties under greenhouse and field conditions were assessed. While peroxidase transcript accumulation was induced in CLas infected sweet orange varieties, peroxidase enzymatic activity varied. Specific serine proteases were upregulated in Washington navel in the presence of CLas based on quantitative proteomics. Subsequent activity-based protein profiling revealed increased activity of two serine proteases, and reduced activity of one protease in two C. sinensis sweet orange varieties under greenhouse and field conditions. The observations in the current study highlight global reprogramming of the citrus vascular proteome and differential regulation of enzyme classes in response to CLas infection. These results open an avenue for further investigation of diverse responses to HLB across different environmental conditions and citrus genotypes. |
| Databáze: | OpenAIRE |
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