Combined and independent cytotoxicity of sodium hypochlorite, ethylenediaminetetraacetic acid and chlorhexidine
| Autor: | F Ziouti, Nikolaos Economides, Triantafyllia Vouzara, Elisabeth A. Koulaouzidou |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Serial dilution Sodium Hypochlorite Inorganic chemistry Ethylenediaminetetraacetic acid Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Toxicity Tests medicine Humans Bovine serum albumin Cytotoxicity General Dentistry IC50 Edetic Acid Growth medium Analysis of Variance Chromatography biology Root Canal Irrigants Chlorhexidine 030206 dentistry 030104 developmental biology chemistry Sodium hypochlorite biology.protein Anti-Infective Agents Local medicine.drug |
| Zdroj: | International endodontic journal. 49(8) |
| ISSN: | 1365-2591 |
| Popis: | Aim To evaluate the capacity of commonly used root canal irrigants to induce cytotoxic effects, when applied singly or in combination. The hypothesis tested was that the irrigants were less cytotoxic when applied in combination than independently. Methodology MRC5 cells were grown as monolayer cultures at 37 °C in an atmosphere containing 5% CO2 in air and 100% relative humidity. Cells were exposed to sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), chlorhexidine (CHX) and their combinations (NaOCl/EDTA, NaOCl/CHX, EDTA/CHX) in serial dilutions. Growth medium was Dulbecco's modified Eagle's medium supplemented with 10% foetal bovine serum and antibiotics and was used as control. The effect on cell survival was estimated after 6 and 24 h of exposure by means of the sulforhodamine B assay, in reference to controls. Dose–response curves were plotted, and 50% inhibitory doses (IC50) were subjected to statistical analysis (anova and post hoc comparison test; P |
| Databáze: | OpenAIRE |
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