Pharmacokinetics of Eight Flavonoids in Rats Assayed by UPLC-MS/MS after Oral Administration of Drynariae rhizoma Extract
| Autor: | Fang Geng, Zhanling Xu, Hai-Tao Wang, Ming-Yang Liu, Mingyue Xu, Qing-Xuan Xu, Chun-Peng Jia, Ning Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Chromatography
lcsh:QD71-142 Article Subject 010405 organic chemistry General Chemical Engineering 010401 analytical chemistry Selected reaction monitoring lcsh:Analytical chemistry 01 natural sciences 0104 chemical sciences Computer Science Applications Analytical Chemistry Triple quadrupole mass spectrometer chemistry.chemical_compound chemistry Pharmacokinetics Neoeriocitrin Astragalin Kaempferol Instrumentation Naringin Luteolin Research Article |
| Zdroj: | Journal of Analytical Methods in Chemistry Journal of Analytical Methods in Chemistry, Vol 2018 (2018) |
| ISSN: | 2090-8865 |
| DOI: | 10.1155/2018/4789196 |
| Popis: | As a traditional Chinese medicine, Drynariae rhizoma (Kunze ex Mett.) J. Sm. has been used to treat osteoporosis and bone resorption for 2500 years. Based on the previous study and literature references, flavonoids were proved to be the most abundant and main active compounds of Drynariae rhizoma for osteoporosis treatment. In order to make good and rational use of Drynariae rhizoma in future, a rapid, sensitive, and selective ultraperformance liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed to investigate the pharmacokinetics of eight main flavonoids in rat plasma after oral administration of the Drynariae rhizoma extract, including neoeriocitrin, luteolin-7-O-β-D-glucoside, astragalin, naringin, eriodictyol, luteolin, naringenin, and kaempferol. Plasma samples’ pretreatment involved a solid-phase extraction column. The separation was performed on an ACQUITY UPLCTM BEH C18 column with a gradient mobile-phase system of acetonitrile and 1% acetic acid in water. The detection was performed using a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization interface (ESI) by multiple reaction monitoring (MRM) in the positive ion mode. All calibration curves exhibited good linearity (r2 > 0.9990) over the measured ranges. The intraday and interday precisions (RSD) were within 13.87%, and the accuracy (RE) ranged from −14.57% to −0.25% at three quality control levels. Extraction recovery, matrix effect, and stability were satisfactory. The pharmacokinetic characteristics of the eight flavonoids of interest were clearly elucidated. |
| Databáze: | OpenAIRE |
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