Contrast-enhancement for the image of human immunodeficiency virus from ultrathin section by immuno electron microscopy
| Autor: | Chizuko Morita, Takehiro Kohno, Shinichi Morimatsu, Toshiyuki Goto, Takashi Nakano, Yoshihiko Fujioka, Kouichi Sano |
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| Rok vydání: | 1998 |
| Předmět: |
Pathology
medicine.medical_specialty Ruthenium red Contrast enhancement Osmium Tetroxide T-Lymphocytes Immunoelectron microscopy Biology Cell Line law.invention chemistry.chemical_compound law Virology Microscopy medicine Humans Microscopy Immunoelectron Immunogold labelling Image Enhancement Immunohistochemistry Ruthenium Red Osmium tetroxide chemistry HIV-2 Ultrastructure Electron microscope Resins Plant Nuclear chemistry |
| Zdroj: | Journal of Virological Methods. 72:137-143 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/s0166-0934(98)00022-6 |
| Popis: | A simple contrast-enhancement method is described for electron microscopic imaging of the human immunodeficiency virus (HIV) from a sample embedded in Lowicryl K4M resin, by immuneelectron microscopy. Ultrathin sections were treated with a mixture of ruthenium red dye (RR) and osmium tetroxide (OsO 4 ). This treatment provided good contrast enhancement of the entire ultrastructural image of virus particles without the loss of immunolabelling. RR/OsO 4 solution is simple to prepare and provides a better contrast than that which is achieved during conventional post-embedding immunoelectron microscopy. Treatment of ultrathin sections from low temperature-embedded samples with RR/OsO 4 solution is recommended. |
| Databáze: | OpenAIRE |
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