A 'GC-rich' method for mammalian gene expression: a dominant role of non-coding DNA GC content in regulation of mammalian gene expression
| Autor: | Jingshuang Wei, Ran Tao, JouDi Aziz, Xiyan Liu, Hui-Cheng Li, Xingjun Zhou, Jun Ma, Wenxian Liu, Matthew Mizhou Hui, Yajun Zuo, XinHui Zhao, Wang Yantao, Xiu-Qin Zhang, ChunHua Huang, Lingling Song, Shuyin Wu, Dong Xiangfeng, Chunling Du, Yang Wei, Zheng-Wu Li, GuanPing Chen, Shuaidong Ye, Yanmin Huang, Luanfeng Li, Bo Li, Ying Zhang, YiGao Song, Qian Jia, LiPing Xing, Weiyuan Jin, Liwen Li, Jian Gao, YuYang Teng, Liang Qiubo, Hongjie Zhu, XueTing Zhang, Chen Wei, Hongtao Wu, Lihua Hou, Lijun Cheng, Wei Zhao, GouQing Leng, Gilbert M. Rishton |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Therapeutic gene modulation
Molecular Sequence Data Gene Expression CHO Cells Biology Transfection General Biochemistry Genetics and Molecular Biology Cricetulus Cricetinae Animals Protein–DNA interaction Gene Cells Cultured General Environmental Science Regulator gene Regulation of gene expression Mammals Base Composition Base Sequence DNA Molecular biology Actins Chromatin Introns Heterologous expression General Agricultural and Biological Sciences Chickens GC-content |
| Zdroj: | Science China. Life sciences. 53(1) |
| ISSN: | 1869-1889 |
| Popis: | High mammalian gene expression was obtained for more than twenty different proteins in different cell types by just a few laboratory scale stable gene transfections for each protein. The stable expression vectors were constructed by inserting a naturally-occurring 1.006 kb or a synthetic 0.733 kb DNA fragment (including intron) of extremely GC-rich at the 5′ or/and 3′ flanking regions of these protein genes or their gene promoters. This experiment is the first experimental evidence showing that a non-coding extremely GC-rich DNA fragment is a super “chromatin opening element” and plays an important role in mammalian gene expression. This experiment has further indicated that chromatin-based regulation of mammalian gene expression is at least partially embedded in DNA primary structure, namely DNA GC-content. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink