Immunomodulatory activity of non starch polysaccharides isolated from green gram (Vigna radiata)
| Autor: | Kiranmayi Ketha, Muralikrishna Gudipati |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Arabinose Ion chromatography 02 engineering and technology Uronic acid Vigna 03 medical and health sciences chemistry.chemical_compound Polysaccharides Animals Immunologic Factors Hemicellulose Food science Rats Wistar Sugar Cells Cultured Cell Proliferation biology Plant Extracts Macrophages 021001 nanoscience & nanotechnology biology.organism_classification Rats 030104 developmental biology chemistry Sodium hydroxide Galactose 0210 nano-technology Spleen Food Science |
| Zdroj: | Food Research International. 113:269-276 |
| ISSN: | 0963-9969 |
| DOI: | 10.1016/j.foodres.2018.07.010 |
| Popis: | Green gram, rich in dietary fiber is known to enhance the function of immune system. However information pertaining to the immunomodulatory potential of its non starch polysaccharides (NSPs) is scanty. Hence, five different NSPs were extracted successively using water (WSP), hot water (55 o C, HWSP), EDTA (0.5%, Pectins) and alkali (10%, Hemicellulose A and B) which varied in their arabinose to galactose ratio, sugar, protein, uronic acid contents, molecular weight distribution and immunomodulatory activity. Hemicellulose B was relatively rich in carbohydrate content (~95%) and also possessed potent immunomodulatory activity among the various NSPs. Hemicellulose B was further fractionated on DEAE-cellulose column into six different fractions by eluting step-wise with water, ammonium carbonate (0.1, 0.2, 0.3 M AC) and sodium hydroxide (0.1 and 0.2 M NaOH). 0.1 M AC eluted fraction was found to be the major one amounting to ~ 50% yield and showed relatively significant (p |
| Databáze: | OpenAIRE |
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