A spatiotemporally regulated transcriptional complex underlies heteroblastic development of leaf hairs in Arabidopsis thaliana

Autor: Hang Ren, Hongbo Tang, Ling‐Zi Li, Yan-Xia Mai, Xiao‐Li Liu, Jian Gao, Lian-Yu Wu, Heng Lian, Jiawei Wang, Fu-Xiang Wang, Guang‐Dong Shang, Er‐Wang Chen, Xue‐Ning Zhang, Chang‐Sheng Wang, Chuan-Miao Zhou, Long Wang, Tian-Qi Zhang, Chang Liu, Lin Han
Rok vydání: 2019
Předmět:
Transcriptional Activation
Arabidopsis
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Spatio-Temporal Analysis
0302 clinical medicine
Gene Expression Regulation
Plant
Arabidopsis thaliana
MYB
Promoter Regions
Genetic
Downstream Enhancer
Molecular Biology
Transcription factor
030304 developmental biology
Homeodomain Proteins
0303 health sciences
Polarity (international relations)
General Immunology and Microbiology
biology
Arabidopsis Proteins
General Neuroscience
fungi
Gene Expression Regulation
Developmental
food and beverages
Articles
Plants
Genetically Modified
biology.organism_classification
Trichome
Chromatin
Cell biology
DNA-Binding Proteins
Plant Leaves
MicroRNAs
Phenotype
Mutation
Chromatin Loop
030217 neurology & neurosurgery
Transcription Factors
Zdroj: The EMBO Journal. 38
ISSN: 1460-2075
0261-4189
DOI: 10.15252/embj.2018100063
Popis: Heteroblasty refers to a phenomenon that a plant produces morphologically or functionally different lateral organs in an age‐dependent manner. In the model plant Arabidopsis thaliana, the production of trichomes (epidermal leaf hairs) on the abaxial (lower) side of leaves is a heteroblastic mark for the juvenile‐to‐adult transition. Here, we show that the heteroblastic development of abaxial trichomes is regulated by a spatiotemporally regulated complex comprising the leaf abaxial fate determinant (KAN1) and the developmental timer (miR172‐targeted AP2‐like proteins). We provide evidence that a short‐distance chromatin loop brings the downstream enhancer element into close association with the promoter elements of GL1, which encodes a MYB transcription factor essential for trichome initiation. During juvenile phase, the KAN1‐AP2 repressive complex binds to the downstream sequence of GL1 and represses its expression through chromatin looping. As plants age, the gradual reduction in AP2‐like protein levels leads to decreased amount of the KAN1‐AP2 complex, thereby licensing GL1 expression and the abaxial trichome initiation. Our results thus reveal a novel molecular mechanism by which a heteroblastic trait is governed by integrating age and leaf polarity cue in plants.
Databáze: OpenAIRE
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