Impact of a Rapid Herpes Simplex Virus PCR Assay on Duration of Acyclovir Therapy
| Autor: | Tam T. Van, Melissa Arevalo, Maryann Lustestica, Jennifer Dien Bard, Kanokporn Mongkolrattanothai |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult Central Nervous System Male medicine.medical_specialty Adolescent viruses 030106 microbiology Acyclovir Herpesvirus 1 Human medicine.disease_cause Real-Time Polymerase Chain Reaction Gastroenterology Antiviral Agents 03 medical and health sciences Young Adult 0302 clinical medicine Cerebrospinal fluid 030225 pediatrics Internal medicine Virology medicine Humans Meningitis Child Cerebrospinal Fluid Retrospective Studies business.industry Mortality rate Infant Newborn Infant Herpes Simplex medicine.disease Discontinuation Herpes simplex virus Specimen collection Child Preschool Toxicity Female Encephalitis Herpes Simplex business Encephalitis |
| Zdroj: | Journal of clinical microbiology. 55(5) |
| ISSN: | 1098-660X |
| Popis: | Herpes simplex virus (HSV) infections of the central nervous system (CNS) are associated with significant morbidity and mortality rates in children. This study assessed the impact of a direct HSV (dHSV) PCR assay on the time to result reporting and the duration of acyclovir therapy for children with signs and symptoms of meningitis and encephalitis. A total of 363 patients with HSV PCR results from cerebrospinal fluid (CSF) samples were included in this retrospective analysis, divided into preimplementation and postimplementation groups. For the preimplementation group, CSF testing was performed using a laboratory-developed real-time PCR assay; for the postimplementation group, CSF samples were tested using a direct sample-to-answer assay. All CSF samples were negative for HSV. Over 60% of patients from both groups were prescribed acyclovir. The average HSV PCR test turnaround time for the postimplementation group was reduced by 14.5 h (23.6 h versus 9.1 h; P < 0.001). Furthermore, 79 patients (43.6%) in the postimplementation group had dHSV PCR results reported P < 0.001). The median duration of acyclovir therapy was also significantly reduced in the postimplementation group (29.2 h versus 14.3 h; P = 0.01). Our investigation suggests that implementation of rapid HSV PCR testing can decrease turnaround times and the duration of unnecessary acyclovir therapy. |
| Databáze: | OpenAIRE |
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