Intracardiac transplantation of skeletal myoblasts yields two populations of striated cells in situ
| Autor: | Doris A. Taylor, Kelley A. Hutcheson, Cleveland W. Lewis, B. Zane Atkins, William E. Kraus, Donald D. Glower |
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| Rok vydání: | 1999 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty Cell Transplantation Biopsy Transplantation Autologous Lesion Multinucleate medicine Animals Regeneration Myocyte Cardiomyoplasty Muscle Skeletal Soleus muscle business.industry Myocardium Skeletal muscle Anatomy Immunohistochemistry Transplantation medicine.anatomical_structure Ventricle Surgery Rabbits Stem cell medicine.symptom Cardiology and Cardiovascular Medicine business |
| Zdroj: | The Annals of Thoracic Surgery. 67:124-129 |
| ISSN: | 0003-4975 |
| DOI: | 10.1016/s0003-4975(98)01197-7 |
| Popis: | Background . Adult heart lacks stem cells and cannot effectively regenerate. In contrast, skeletal muscle is constantly undergoing repair. We proposed to transplant immature skeletal myoblasts into injured myocardium. Methods . Approximately 7 × 10 6 soleus skeletal myoblasts were expanded in vitro from adult New Zealand White rabbits (n = 23) whose posterior left ventricle was cryoinjured to create a transmural lesion. Autologous myoblasts (n = 18) or saline (n = 5) was transplanted into the central cryolesion at the time of injury (n = 6) or 1 week later (n = 12). Hearts were harvested 2 weeks after injection. Results . Myoblast transfer did not incur further morbidity. After cryolesion, grossly, a 1.6-cm epicardial hemorrhagic lesion could be seen. Histologically, the transmural lesion contained inflammatory cells and active scarring but no viable cardiomyocytes. Electron microscopy demonstrated a predominance of collagen and fibroblasts. Nine hearts contained multinucleated cells within the cryolesion that covered approximately 75% of the central cryolesion in 17% of animals. Immunohistochemical analysis confirmed their skeletal muscle origin. At the periphery of the lesion, isolated clusters of nonskeletal muscle cells could be visualized (n = 12) that resembled immature cardiocytes. Conclusions . Autologous skeletal myoblasts can regenerate viable striated tissue within damaged myocardium. Myoblast transfer warrants further investigation as a new method for improving myocardial performance within infarcted myocardium. |
| Databáze: | OpenAIRE |
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