A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages
| Autor: | Jung-Seok Kim, Yuan Xia, Steffen Jung, Sébastien Trzebanski, Sigalit Boura-Halfon, Isabelle Scheyltjens, Kiavash Movahedi, Louise Chappell-Maor, Hannah Van Hove, Zhana Haimon, Shany Peled-Hajaj, Anat Shemer, Masha Kolesnikov, Alisa Lubart, Pablo Blinder |
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| Přispěvatelé: | Department of Bio-engineering Sciences, Faculty of Sciences and Bioengineering Sciences, Basic (bio-) Medical Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Central Nervous System Cell type binary transgenic Transgene Neuroscience(all) Immunology Cell Mutagenesis (molecular biology technique) microglia pia mater Mice Transgenic Biology 03 medical and health sciences Mice 0302 clinical medicine split cre meninges Immunology and Microbiology(all) Gene expression medicine Recombinase Lyve1 BAM brain macrophages Immunology and Allergy Animals Cells Cultured Mice Knockout Microglia Integrases Macrophages Brain intersectional genetics Cell biology Transgenesis Mice Inbred C57BL 030104 developmental biology Infectious Diseases medicine.anatomical_structure Organ Specificity 030220 oncology & carcinogenesis RiboTag perivascular macrophages |
| DOI: | 10.1016/j.immuni.2020.11.007 |
| Popis: | The developmental and molecular heterogeneity of tissue macrophages is unravelling, as are their diverse contributions to physiology and pathophysiology. Moreover, also given tissues harbor macrophages in discrete anatomic locations. Functional contributions of specific cell populations can in mice be dissected using Cre recombinase-mediated mutagenesis. However, single promoter-based Cre models show limited specificity for cell types. Focusing on macrophages in the brain, we establish here a binary transgenic system involving complementation-competent NCre and CCre fragments whose expression is driven by distinct promoters: Sall1ncre: Cx3cr1ccre mice specifically target parenchymal microglia and compound transgenic Lyve1ncre: Cx3cr1ccre animals target vasculature-associated macrophages, in the brain, as well as other tissues. We imaged the respective cell populations and retrieved their specific translatomes using the RiboTag in order to define them and analyze their differential responses to a challenge. Collectively, we establish the value of binary transgenesis to dissect tissue macrophage compartments and their functions. |
| Databáze: | OpenAIRE |
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