Stability of Escherichia coli Transcription Complexes Near an Intrinsic Terminator
| Autor: | P H von Hippel, Kevin S. Wilson |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Hot Temperature
Transcription Genetic Termination factor Molecular Sequence Data Molecular Conformation chemistry.chemical_compound Structural Biology Transcription (biology) RNA polymerase Intrinsic termination Escherichia coli RNA Messenger Molecular Biology Polymerase Terminator Regions Genetic Base Sequence Models Genetic biology Nucleotides Chromosome Mapping RNA DNA-Directed RNA Polymerases Bacteriophage lambda Terminator (genetics) Models Chemical Biochemistry chemistry Antitermination Biophysics biology.protein Thermodynamics |
| Zdroj: | Journal of Molecular Biology. 244:36-51 |
| ISSN: | 0022-2836 |
| DOI: | 10.1006/jmbi.1994.1702 |
| Popis: | Transcription complexes containing Escherichia coli RNA polymerase terminate RNA synthesis in response to intrinsic (rho-independent) terminators encoded in the DNA template. This type of termination involves the formation of a hairpin structure in the nascent RNA, leading to release of the RNA and polymerase from the DNA template. In this paper we show that RNA release occurs only within a zone extending over six template positions of the tR2 terminator of phage lambda. The upstream end of this zone is defined by a large and abrupt decrease in the stability of the transcription complex, and this decrease is reversed at the downstream end. Except within this termination zone, RNA chain elongation is completely processive at all template positions, over a wide range of temperatures (20 to 70 degrees). The stability of ternary complexes halted at each template position by the incorporation of 3'-deoxynucleotide substrate analogues was determined by means of an ultrafiltration RNA release assay. This assay shows that the nascent RNA is released from halted ternary complexes within the same zone in which termination occurs during active transcription. The termination efficiency at each position within the tR2 termination zone is a sensitive function of the concentration of the next nucleotide substrate to be incorporated into the nascent RNA. These results suggest that termination positions are defined by the thermodynamic stability of the polymerase complex, while the efficiencies of termination are determined by a kinetic competition between RNA elongation and release at each position. Based on a kinetic competition model, the temperature dependence of the efficiency indicates that the elongation pathway is favored enthalpically, while the termination pathway is favored entropically. |
| Databáze: | OpenAIRE |
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