Characterization of a novel flooding stress-responsive alcohol dehydrogenase expressed in soybean roots
| Autor: | Setsuko, Komatsu, Thibaut, Deschamps, Deschamps, Thibaut, Susumu, Hiraga, Mikio, Kato, Mitsuru, Chiba, Akiko, Hashiguchi, Makoto, Tougou, Satoshi, Shimamura, Hiroshi, Yasue |
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| Rok vydání: | 2011 |
| Předmět: |
Time Factors
Immunoblotting Molecular Sequence Data Plant Science In situ hybridization Sodium Chloride Biology Plant Roots Gene Expression Regulation Plant Stress Physiological parasitic diseases Gene expression Genetics Electrophoresis Gel Two-Dimensional Amino Acid Sequence Hypoxia In Situ Hybridization Southern blot Alcohol dehydrogenase Regulation of gene expression Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling fungi Alcohol Dehydrogenase Water food and beverages General Medicine Meristem Floods Hypocotyl Cold Temperature Gene expression profiling Biochemistry Seedlings Soybean Proteins biology.protein Soybeans Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology. 77:309-322 |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/s11103-011-9812-y |
| Popis: | Alcohol dehydrogenase (Adh) is the key enzyme in alcohol fermentation. We analyzed Adh expression in order to clarify the role of Adh of soybeans (Glycine max) to flooding stress. Proteome analysis confirmed that expression of Adh is significantly upregulated in 4-day-old soybean seedlings subjected to 2 days of flooding. Southern hybridization analysis and soybean genome database search revealed that soybean has at least 6 Adh genes. The GmAdh2 gene that responded to flooding was isolated from soybean cultivar Enrei. Adh2 expression was markedly increased 6 h after flooding and decreased 24 h after floodwater drainage. In situ hybridization and Western blot indicated that flooding strongly induces Adh2 expression in RNA and protein levels in the root apical meristem. Osmotic, cold, or drought stress did not induce expression of Adh2. These results indicate that Adh2 is a flooding-response specific soybean gene expressed in root tissue. |
| Databáze: | OpenAIRE |
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