Incorporation of NS1 and prM/M are important to confer effective protection of adenovirus-vectored Zika virus vaccine carrying E protein
| Autor: | Shengnan Zhang, Qihong Yan, Heying Li, Yupeng Feng, X.L. Ye, Pingchao Li, Zhipeng Yao, Ling Chen, Mingli Hao, Changhua Yi, Ruian Xu, Xuefeng Niu, Linbing Qu, Feng Li, Xuehua Zheng, Wan Su, Peihai Chen, Xinglong Liu, Liqiang Feng, Qian Wang, Weiqi Pan |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:Immunologic diseases. Allergy viruses Immunology Biology Immune sera Brief Communication lcsh:RC254-282 Zika virus 03 medical and health sciences 0302 clinical medicine Viral inhibition Pharmacology (medical) Neutralizing antibody Target antigen Pharmacology virus diseases biochemical phenomena metabolism and nutrition biology.organism_classification lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Virology stomatognathic diseases 030104 developmental biology Infectious Diseases Viral replication biology.protein lcsh:RC581-607 030215 immunology |
| Zdroj: | npj Vaccines, Vol 3, Iss 1, Pp 1-8 (2018) NPJ Vaccines |
| ISSN: | 2059-0105 |
| DOI: | 10.1038/s41541-018-0072-6 |
| Popis: | Current design of Zika virus (ZIKV) vaccine mainly considered envelope (E) as the major target antigen. Non-structural protein NS1 was seldom considered. Herein, we generated three adenovirus-vectored vaccines carrying E (Ad2-E), or premembrane/membrane (prM/M) with E (Ad2-prME), or NS1 in addition to prM/M with E (Ad2-prME-NS1). Ad2-prME induced higher neutralizing antibody response to ZIKV than Ad2-E, suggesting prM/M is important for the folding of immunogenic E. Most intriguingly, Ad2-prME-NS1 elicited the best viral inhibition when the immune sera were added to ZIKV-infected cells. In ZIKV-challenged neonatal mice born to maternally immunized dams, Ad2-prME-NS1 conferred the best protection in preventing weight loss, neurological disorders, and viral replication. Ad2-prME also conferred significant protection but was less effective than Ad2-prME-NS1, whereas Ad2-E only alleviated neurological symptoms but did not inhibit viral replication. Our study suggested that NS1 should be considered in the design of ZIKV vaccine in addition to prM/M and E. |
| Databáze: | OpenAIRE |
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