Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells
| Autor: | Sharon Dell, Alice Meunier, Rashmi Nanjundappa, Zhen Liu, Quynh P. H. Nguyen, Moe R. Mahjoub, Regan Doherty, Kirk J. Czymmek, Vito Mennella, Alexandre Megherbi, Claire L. Jackson, Yew M. Heng, Nathalie Delgehyr, James Thompson, Jane S. Lucas, Alexandra Albulescu |
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| Rok vydání: | 2019 |
| Předmět: |
Centriole
Mucociliary clearance Biology General Biochemistry Genetics and Molecular Biology Article Epithelium 03 medical and health sciences 0302 clinical medicine medicine Basal body Humans Cilia Molecular Biology Cells Cultured 030304 developmental biology Primary ciliary dyskinesia Centrioles 0303 health sciences Microscopy Cilium Cell Differentiation Epithelial Cells Cell Biology respiratory system medicine.disease Basal Bodies Cell biology medicine.anatomical_structure Centrosome Motile cilium 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Dev Cell |
| ISSN: | 1878-1551 |
| Popis: | Motile cilia are cellular beating machines that play a critical role in mucociliary clearance, cerebrospinal fluid movement, and fertility. In the airways, hundreds of motile cilia present on the surface of a multiciliated epithelia cell beat coordinately to protect the epithelium from bacteria, viruses, and harmful particulates. During multiciliated cell differentiation, motile cilia are templated from basal bodies, each extending a basal foot-an appendage linking motile cilia together to ensure coordinated beating. Here, we demonstrate that among the many motile cilia of a multiciliated cell, a hybrid cilium with structural features of both primary and motile cilia is harbored. The hybrid cilium is conserved in mammalian multiciliated cells, originates from parental centrioles, and its cellular position is biased and dependent on ciliary beating. Furthermore, we show that the hybrid cilium emerges independently of other motile cilia and functions in regulating basal body alignment. |
| Databáze: | OpenAIRE |
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