| Autor: |
Makhoul, Stephanie, Trabold, Katharina, Gambaryan, Stepan, Tenzer, Stefan, Pillitteri, Daniele, Walter, Ulrich, Jurk, Kerstin |
| Rok vydání: |
2019 |
| DOI: |
10.6084/m9.figshare.9830303 |
| Popis: |
Figure S8. Iloprost and riociguat inhibit convulxin-induced platelet aggregation but not Syk activation. Washed human platelets (WP) were pre-incubated with iloprost (2 nM, 3 min) or riociguat (20 μM, 2 min) prior to stimulation with 50 ng/ml convulxin. a Representative aggregation curves and the corresponding quantitative data are shown in b. c Aggregation was stopped after 1, 2 or 5 min using Laemmli buffer. Syk Y525/526 and Y352 were analyzed by western blot. d, e Quantification of the phosphorylated proteins is presented as ratio compared to the total Syk protein. Results are representative of at least 3 different experiments with platelets from at least 3 healthy volunteers, data are presented as means ± S.D. ns: not significant, ****p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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