Comparison of the intestinal microbiome of italian patients with multiple sclerosis and their household relatives
| Autor: | Domenico Nuzzo, Luca Scalisi, Fanny Claire Capri, Sabrina Realmuto, Luca Vecchioni, Salvatore Cottone, Rosa Alduina, Paola Galluzzo |
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| Přispěvatelé: | Galluzzo P., Capri F.C., Vecchioni L., Realmuto S., Scalisi L., Cottone S., Nuzzo D., Alduina R. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Ruminococcaceae
Bacteroidaceae Science Veillonellaceae Disease Gut flora General Biochemistry Genetics and Molecular Biology Article Multiple sclerosis medicine Microbiome Desulfovibrionaceae christensenellaceae Ecology Evolution Behavior and Systematics Genetics Clostridiales biology Burkholderiaceae Paleontology biology.organism_classification medicine.disease Space and Planetary Science Tannerellaceae |
| Zdroj: | Life Volume 11 Issue 7 Life, Vol 11, Iss 620, p 620 (2021) Life (Basel) (2021). doi:10.3390/life11070620 info:cnr-pdr/source/autori:Paola Galluzzo; Fanny Claire Capri; Luca Vecchioni; Sabrina Realmuto; Luca Scalisi; Salvatore Cottone; Domenico Nuzzo; Rosa Alduina/titolo:Comparison of the Intestinal Microbiome of Italian Patients with Multiple Sclerosis and Their Household Relatives/doi:10.3390%2Flife11070620/rivista:Life (Basel)/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| DOI: | 10.3390/life11070620 |
| Popis: | Multiple sclerosis (MS) is a chronic immune-mediated disease of the central nervous system, caused by a combination of genetic and environmental factors. In recent years, a role in MS pathogenesis was assigned to the gut microbiota. However, different signatures of gut dysbiosis have been shown to depend on environmental factors, like diet and lifestyle. In this study, we compared the gut microbiome in MS patients and their household healthy relatives sharing lifestyle and environmental factors. Faecal metagenomic DNA was extracted and the V3–V4 regions of the conserved bacterial 16S ribosomal RNA gene were amplified and sequenced. While overall bacterial communities were similar, specific families differed between healthy and MS subjects. We observed an increase in Ruminococcaceae, Christensenellaceae, Desulfovibrionaceae, Clostridiales, and Family XIII in MS patients, while Bacteroidaceae, Tannerellaceae, Veillonellaceae, and Burkholderiaceae were more abundant in healthy controls. In addition, principle coordinate analysis showed that the gut microbiome of all MS patients formed a cluster being less diverse than the household relatives and that gut microbiota of MS patients with EDSS 4.5–7 formed a distinct cluster in respect to their controls. Overall, our study is consistent with the hypothesis that MS patients have gut microbial dysbiosis and evidenced the importance of environmental factors in shaping the gut microbiome. |
| Databáze: | OpenAIRE |
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