A Novel Model of Severe Gallstone Pancreatitis: Murine Pancreatic Duct Ligation Results in Systemic Inflammation and Substantial Mortality
| Autor: | Zuobiao Yuan, Erik Twait, Isaac Samuel, Duraisamy Kempuraj, David K. Meyerholz, Deborah E. Williard |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Pathology Endocrinology Diabetes and Metabolism Gastroenterology Mice Internal medicine Acinar cell Animals Medicine Ligation Lung Compliance Common Bile Duct Inflammation Pancreatic duct Hepatology Common bile duct business.industry Bile duct Pancreatic Ducts medicine.disease Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Pancreatitis Acute pancreatitis business Multiple organ dysfunction syndrome Rapid Communication |
| Zdroj: | Pancreatology. 10:536-544 |
| ISSN: | 1424-3903 |
| DOI: | 10.1159/000320776 |
| Popis: | Suitable experimental models of gallstone pancreatitis with systemic inflammation and mortality are limited. We developed a novel murine model of duct-ligation-induced acute pancreatitis associated with multiorgan dysfunction and severe mortality.Laparotomy was done on C57/BL6 mice followed by pancreatic duct (PD) ligation, bile duct (BD) ligation without PD ligation, or sham operation.Only mice with PD ligation developed acute pancreatitis and had 100% mortality. Pulmonary compliance was significantly reduced after PD ligation but not BD ligation. Bronchoalveolar lavage fluid neutrophil count and interleukin-1β concentration, and the plasma creatinine level, were significantly elevated with PD ligation but not BD ligation. Pancreatic nuclear factor κB (p65) and activator protein 1 (c-Jun) were activated within 1 h of PD ligation.PD-ligation-induced acute pancreatitis in mice is associated with systemic inflammation, acute lung injury, multiorgan dysfunction and death. The development of this novel model is an exciting and notable advance in the field. |
| Databáze: | OpenAIRE |
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