Beauveria bassiana Xylanase: Characterization and Wastepaper Deinking Potential of a Novel Glycosyl Hydrolase from an Endophytic Fungal Entomopathogen
| Autor: | Prashant K. Bhagwat, Santhosh Pillai, Suren Singh, Ayodeji Amobonye |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
QH301-705.5 enzymes Beauveria bassiana Plant Science Bassiana Endophyte Article chemistry.chemical_compound Hydrolase Glycosyl Biology (General) Ecology Evolution Behavior and Systematics xylanase biology Chemistry fungi biology.organism_classification entomopathogen Enzyme assay deinking Biochemistry Entomopathogenic fungus Xylanase biology.protein alternative application endophyte |
| Zdroj: | Journal of Fungi Volume 7 Issue 8 Journal of Fungi, Vol 7, Iss 668, p 668 (2021) |
| ISSN: | 2309-608X |
| Popis: | Beauveria bassiana is an entomopathogenic fungus widely used as a biopesticide for insect control it has also been shown to exist as an endophyte, promoting plant growth in many instances. This study highlights an alternative potential of the fungus in the production of an industrially important biocatalyst, xylanase. In this regard, Beauveria bassiana SAN01 xylanase was purified to homogeneity and subsequently characterized. The purified xylanase was found to have a specific activity of 324.2 U·mg−1 and an estimated molecular mass of ~37 kDa. In addition, it demonstrated optimal activity at pH 6.0 and 45 °C while obeying Michaelis–Menton kinetics towards beechwood xylan with apparent Km, Vmax and kcat of 1.98 mg·mL−1, 6.65 μM·min−1 and 0.62 s−1 respectively. The enzyme activity was strongly inhibited by Ag2+ and Fe3+ while it was significantly enhanced by Co2+ and Mg2+. Furthermore, the xylanase was shown to effectively deink wastepaper at an optimal rate of 106.72% through its enzymatic disassociation of the fiber-ink bonds as demonstrated by scanning electron microscopy and infrared spectroscopy. This is the first study to demonstrate the biotechnological application of a homogeneously purified glycosyl hydrolase from B. bassiana. |
| Databáze: | OpenAIRE |
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