Regulation of human cytolytic lymphocyte responses by interleukin-12
| Autor: | Phyllis M. Quinn, Maurice K. Gately, Aimee G. Wolitzky, Richard Anthony Chizzonite |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_treatment
Lymphocyte Immunology chemical and pharmacologic phenomena Biology Lymphocyte Activation Interferon-gamma medicine Humans Killer Cells Lymphokine-Activated Cells Cultured Lymphokine-activated killer cell Tumor Necrosis Factor-alpha Interleukins Interleukin hemic and immune systems T lymphocyte Interleukin-12 CTL Cytolysis Cytokine medicine.anatomical_structure Interleukin 12 Cancer research Interleukin-2 T-Lymphocytes Cytotoxic |
| Zdroj: | Cellular Immunology. 143:127-142 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/0008-8749(92)90011-d |
| Popis: | IL-12 is a heterodimeric cytokine which has been shown to cause the proliferation of activated T and NK cells, to enhance the lytic activity of NK cells, and to induce IFN-gamma production by resting and activated T and NK cells. We previously reported that IL-12 could synergize with IL-2 to activate human LAK cells in the presence of hydrocortisone but that IL-12 alone was inactive. We herein show that in the absence of hydrocortisone, IL-12 by itself can activate human LAK cells. IL-12-induced LAK cell activity was mediated predominantly by CD56+ lymphocytes. Activation of LAK cells by IL-12 appeared to be independent of IL-2 since it was not inhibited by neutralizing anti-human IL-2. However, IL-12- and IL-2-induced LAK cell activation could be partially inhibited by anti-human TNF-alpha. Moreover, IL-12 produced in situ appeared to play a role in IL-2-induced LAK cell activation since rat monoclonal antibodies to human IL-12 could partially inhibit the generation of LAK cells in response to IL-2. In addition to its effects on LAK cell responses, IL-12 could facilitate specific allogeneic human CTL responses. However, IL-12-facilitated CTL responses were blocked by neutralizing anti-human IL-2 indicating a requirement for IL-2 produced in situ. The ability of IL-12 to facilitate both nonspecific LAK and specific CTL responses suggests that it may be useful as a therapeutic agent against some tumors and infectious diseases. |
| Databáze: | OpenAIRE |
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