Competitive immunochromatographic assay for leptosperin as a plausible authentication marker of manuka honey
| Autor: | Akari Ishisaka, Toshio Niwa, Yosuke Takimoto, Noritoshi Kitamoto, Maki Juri, Yoko Nitta, Yukako Araki, Yoji Kato |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Quality Control
Methyl syringate Beekeeping Chromatography Chemistry digestive oral and skin physiology 010401 analytical chemistry food and beverages Honey 04 agricultural and veterinary sciences General Medicine 040401 food science 01 natural sciences Honey samples High-performance liquid chromatography Chromatography Affinity Manuka Honey 0104 chemical sciences Analytical Chemistry Leptospermum 0404 agricultural biotechnology Glycosides Antibacterial activity Food Science |
| Zdroj: | Food Chemistry. 194:362-365 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2015.08.040 |
| Popis: | Manuka honey is known as one of the premium honeys because of its unique property: a potent antibacterial activity. Leptosperin, methyl syringate 4-O-β-d-gentiobioside, has been specifically identified in manuka honey. Because leptosperin is relatively stable under warmer conditions, measuring leptosperin levels may be applied to authenticate manuka honey. In this study, an immunochromatographic separation and quantification of leptosperin techniques have been developed. The concentration of leptosperin measured by immunochromatography was significantly correlated with the concentration measured by high-performance liquid chromatography (HPLC) or enzyme-linked immunosorbent assay (ELISA). Because the immunochromatographic method is rapid and reliable, it could be applied to on-site quality control or inspection of honey samples by a beekeeper, a manufacturer, an inspector, a retailer, or a consumer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect