Tooth Removal in the Leopard Gecko and the de novo Formation of Replacement Teeth
| Autor: | Joy M. Richman, Theresa M. Grieco, Kirstin S. Brink, Katherine Fu, Jesus Rodolfo Martin Del Campo, Joaquín Ignacio Henríquez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Cell type
Pathology medicine.medical_specialty Physiology Population Polyphyodont Biology dentition pulse-chase 03 medical and health sciences 0302 clinical medicine stomatognathic system Physiology (medical) medicine QP1-981 education 030304 developmental biology Original Research 0303 health sciences education.field_of_study Enamel paint Dentition biology.organism_classification Dental lamina label-retaining cell polyphyodont reptile dental epithelium stomatognathic diseases adult tissue stem cells visual_art Leopard gecko visual_art.visual_art_medium Stem cell 030217 neurology & neurosurgery successional teeth |
| Zdroj: | Frontiers in Physiology Frontiers in Physiology, Vol 12 (2021) |
| ISSN: | 1664-042X |
| Popis: | Many reptiles are able to continuously replace their teeth through life, an ability attributed to the existence of epithelial stem cells. Tooth replacement occurs in a spatially and temporally regulated manner, suggesting the involvement of diffusible factors, potentially over long distances. Here, we locally disrupted tooth replacement in the leopard gecko (Eublepharis macularius) and followed the recovery of the dentition. We looked at the effects on local patterning and functionally tested whether putative epithelial stem cells can give rise to multiple cell types in the enamel organs of new teeth. Second generation teeth with enamel and dentine were removed from adult geckos. The dental lamina was either left intact or disrupted in order to interfere with local patterning cues. The dentition began to reform by 1 month and was nearly recovered by 2–3 months as shown in μCT scans and eruption of teeth labeled with fluorescent markers. Microscopic analysis showed that the dental lamina was fully healed by 1 month. The deepest parts of the dental lamina retained odontogenic identity as shown by PITX2 staining. A pulse-chase was carried out to label cells that were stimulated to enter the cell cycle and then would carry BrdU forward into subsequent tooth generations. Initially we labeled 70–78% of PCNA cells with BrdU. After a 1-month chase, the percentage of BrdU + PCNA labeled cells in the dental lamina had dropped to 10%, consistent with the dilution of the label. There was also a population of single, BrdU-labeled cells present up to 2 months post surgery. These BrdU-labeled cells were almost entirely located in the dental lamina and were the likely progenitor/stem cells because they had not entered the cell cycle. In contrast fragmented BrdU was seen in the PCNA-positive, proliferating enamel organs. Homeostasis and recovery of the gecko dentition was therefore mediated by a stable population of epithelial stem cells in the dental lamina. |
| Databáze: | OpenAIRE |
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