Molecular mechanisms of azole resistance in Candida bloodstream isolates
| Autor: | Robyn Su May Lim, Yiying Cai, Andrea L. Kwa, Jocelyn Qi-Min Teo, Shannon Jing-Yi Lee, Ai-Ling Tan, Tze-Peng Lim |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antifungal Azoles Surveillance study Antifungal Agents medicine.drug_class 030106 microbiology Azole resistance Microbial Sensitivity Tests Biology Microbiology lcsh:Infectious and parasitic diseases Fungal Proteins 03 medical and health sciences 0302 clinical medicine Drug Resistance Fungal Gene Expression Regulation Fungal Candida albicans medicine Humans lcsh:RC109-216 030212 general & internal medicine Gene Fluconazole Candida chemistry.chemical_classification Gene Expression Profiling Candidemia Antifungal resistance Genomics Corpus albicans Amino acid Infectious Diseases chemistry Amino Acid Substitution Candida spp Azole Research Article |
| Zdroj: | BMC Infectious Diseases BMC Infectious Diseases, Vol 19, Iss 1, Pp 1-4 (2019) |
| ISSN: | 1471-2334 |
| Popis: | Background Antifungal resistance rates are increasing. We investigated the mechanisms of azole resistance of Candida spp. bloodstream isolates obtained from a surveillance study conducted between 2012 and 2015. Methods Twenty-six azole non-susceptible Candida spp. clinical isolates were investigated. Antifungal susceptibilities were determined using the Sensititre YeastOne® YO10 panel. The ERG11 gene was amplified and sequenced to identify amino acid polymorphisms, while real-time PCR was utilised to investigate the expression levels of ERG11, CDR1, CDR2 and MDR1. Results Azole cross-resistance was detected in all except two isolates. Amino acid substitutions (A114S, Y257H, E266D, and V488I) were observed in all four C. albicans tested. Of the 17 C. tropicalis isolates, eight (47%) had ERG11 substitutions, of which concurrent observation of Y132F and S154F was the most common. A novel substitution (I166S) was detected in two of the five C. glabrata isolates. Expression levels of the various genes differed between the species but CDR1 and CDR2 overexpression appeared to be more prominent in C. glabrata. Conclusions There was interplay of various different mechanisms, including mechanisms which were not studied here, responsible for azole resistance in Candida spp in our study. |
| Databáze: | OpenAIRE |
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