Nature of DNA polymorphism in the direct repeat cluster of Mycobacterium tuberculosis; application for strain differentiation by a novel typing method
| Autor: | Bunschoten Ae, Peter M.A. Groenen, van Embden Jd, van Soolingen D |
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| Rok vydání: | 1993 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data DNA Ribosomal Polymerase Chain Reaction Microbiology Mycobacterium tuberculosis Spacer Oligonucleotide Typing Species Specificity Genotype Direct repeat Insertion sequence Molecular Biology DNA Primers Repetitive Sequences Nucleic Acid Gene Rearrangement Recombination Genetic Genetics Polymorphism Genetic Base Sequence biology Genetic Variation Gene rearrangement biology.organism_classification Mycobacterium bovis Bacterial Typing Techniques Mycobacterium tuberculosis complex Chromosomal region DNA Transposable Elements Oligonucleotide Probes |
| Zdroj: | Molecular Microbiology. 10:1057-1065 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1993.tb00976.x |
| Popis: | Summary Mycobacterium tuberculosis complex strains contain a unique chromosomal region, which consists of multiple 36bp direct repeats (DRs), which are interspersed by unique spacers 35 to 41 bp in length. In this study we investigated the nature of the DNA polymorphism of this DR cluster by sequencing part of this region in a large number of M. tuberculosis complex strains. Two types of genetic rearrangements were observed. One type consists of the variation in one or a few discrete, contiguous DRs plus spacer sequences. This variation is probably driven by homologous recombination between adjacent or distant DRs. The other type of polymorphism is probably driven by transpositional events of the insertion sequence, IS6110, which is almost invariably present in the DR cluster of M. tuberculosis complex strains. Based on the nature of the DNA polymorphism in the DR cluster, we developed a novel method of strain differentiation, direct variable repeat polymer chain reaction (DVR-PCR), which enables typing of individual M. tuberculosis strains in a single PCR. The method allows an excellent differentiation of epidemiologically unrelated isolates and, in principle, the DVR-PCR allows the detection of M. tuberculosis and strain differentiation at the same time. |
| Databáze: | OpenAIRE |
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