Studies on long chaincis- andTrans-acyl-CoA esters and acyl-CoA dehydrogenase from rat heart mitochondria
| Autor: | Henry B S Conacher, G. A. Jarvis, Joyce L. Beare-Rogers, G. O. Korsrud |
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| Rok vydání: | 1977 |
| Předmět: |
Male
Stereochemistry Dehydrogenase Mitochondrion Biochemistry Michaelis–Menten kinetics Structure-Activity Relationship Acyl-CoA chemistry.chemical_compound Isomerism Animals Coenzyme A biology Chemistry Myocardium Acyl-CoA Dehydrogenase Long-Chain Organic Chemistry Substrate (chemistry) Acyl CoA dehydrogenase Cell Biology Mitochondria Muscle Rats Kinetics Erucic acid Fatty Acids Unsaturated biology.protein Lipidology |
| Zdroj: | Lipids. 12:177-181 |
| ISSN: | 1558-9307 0024-4201 |
| Popis: | The beta-oxidation of long chain fatty acids was investigated in a preparation of rat heart mitochondria. The acyl-CoA esters of the cis and trans isomers of delta9-hexadecenoic, delta9-octadecenoic, delta11-eicosenoic, and delta13-docosenoic acids were prepared. Rates of the acyl-CoA reaction were determined with an extract from rat heart mitochondria. The apparent Michaelis constant (Km) and maximum velocity (Vmax) were calculated for each substrate. In general, apparent Vmax values decreased with increasing chain length of the monoenoic substrates. Reduced activity of acyl-CoA dehydrogenase with long chain acyl-CoA esters could have contributed to accumulation of lipids in hearts of rats fed diets containing long chain fatty acids. |
| Databáze: | OpenAIRE |
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