Blocking connexin channels improves embryo development of vitrified bovine blastocysts†
| Autor: | Ann Van Soom, Mario Van Poucke, Nerea Ortiz-Escribano, Etienne Van den Abbeel, Katarzyna Joanna Szymańska, Elke Decrock, Mélissa Bol, Luc Peelman, Luc Leybaert, Lynn Vandenberghe |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Programmed cell death Hydrostatic pressure Embryonic Development Connexin Biology Connexins Embryo Culture Techniques 03 medical and health sciences medicine Animals Humans Blastocyst Cryopreservation Embryogenesis Gap junction Embryo Cell Biology General Medicine Oocyte Vitrification Cell biology 030104 developmental biology medicine.anatomical_structure Reproductive Medicine embryonic structures Cattle sense organs HeLa Cells |
| Zdroj: | Biology of Reproduction. 96:288-301 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.116.144121 |
| Popis: | Connexins (Cxs) are required for normal embryo development and implantation. They form gap junctions (GJs) connecting the cytoplasm of adjacent cells and hemichannels (HCs), which are normally closed but open in response to stress conditions. Excessive HC opening is detrimental for cell function and may lead to cell death. We found that hatching of in vitro-produced bovine embryos, matured in serum-containing conditions, was significantly improved when vitrification/warming was done in the presence of Gap26 that targets GJA1 (Cx43) and GJA4 (Cx37). Further work showed that HCs from blastocysts produced after oocyte maturation in the presence of serum were open shortly after vitrification/warming, and this was prevented by Gap26. Gap26, applied for the exposure times used, inhibited Cx43 and Cx37 HCs while it did not have an effect on GJs. Interestingly, Gap26 had no effect on blastocyst degeneration or cell death. We conclude that blocking HCs protects embryos during vitrification and warming by a functional effect not linked to cell death. |
| Databáze: | OpenAIRE |
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