Dynamics of the HD regulatory subdomain of PARP-1; substrate access and allostery in PARP activation and inhibition
| Autor: | Marie-France Langelier, Michael McCauley, Elizabeth Underwood, Laura E. Easton, Ji-Chun Yang, David Neuhaus, Kevin J. Embrey, Philip B. Rawlins, John M. Pascal, Marianne Schimpl, Tom E. H. Ogden |
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| Rok vydání: | 2021 |
| Předmět: |
Models
Molecular AcademicSubjects/SCI00010 DNA repair DNA damage Poly ADP ribose polymerase Allosteric regulation Mutant Poly (ADP-Ribose) Polymerase-1 Poly(ADP-ribose) Polymerase Inhibitors 010402 general chemistry Crystallography X-Ray 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Allosteric Regulation Protein Domains Structural Biology Catalytic Domain Genetics 030304 developmental biology 0303 health sciences Substrate Interaction biology Active site Amides 0104 chemical sciences 3. Good health Enzyme Activation chemistry 030220 oncology & carcinogenesis Mutation Biophysics biology.protein DNA DNA Damage |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | PARP-1 is a key early responder to DNA damage in eukaryotic cells. An allosteric mechanism links initial sensing of DNA single-strand breaks by PARP-1’s F1 and F2 domains via a process of further domain assembly to activation of the catalytic domain (CAT); synthesis and attachment of poly(ADP-ribose) (PAR) chains to protein sidechains then signals for assembly of DNA repair components. A key component in transmission of the allosteric signal is the HD subdomain of CAT, which alone bridges between the assembled DNA-binding domains and the active site in the ART subdomain of CAT. Here we present a study of isolated CAT domain from human PARP-1, using NMR-based dynamics experiments to analyse WT apo-protein as well as a set of inhibitor complexes (with veliparib, olaparib, talazoparib and EB-47) and point mutants (L713F, L765A and L765F), together with new crystal structures of the free CAT domain and inhibitor complexes. Variations in both dynamics and structures amongst these species point to a model for full-length PARP-1 activation where first DNA binding and then substrate interaction successively destabilise the folded structure of the HD subdomain to the point where its steric blockade of the active site is released and PAR synthesis can proceed. |
| Databáze: | OpenAIRE |
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