A popular fermented soybean food of Northeast India exerted promising antihyperglycemic potential via stimulating PI3K/AKT/AMPK/GLUT4 signaling pathways and regulating muscle glucose metabolism in type 2 diabetes

Autor:	Dibyendu Das, Sanjib Sarkar, Anjum Dihingia, Nazim Uddin Afzal, Sawlang Borsingh Wann, Jatin Kalita, Saikat Dewanjee, Prasenjit Manna
Rok vydání:	2022
Předmět:	Pharmacology Glucose Transporter Type 4 Muscles Biophysics India Cell Biology AMP-Activated Protein Kinases Isoflavones Rats Diabetes Mellitus Experimental Phosphatidylinositol 3-Kinases Glucose Diabetes Mellitus Type 2 Hyperglycemia Humans Animals Hypoglycemic Agents Soybeans Proto-Oncogene Proteins c-akt Food Science Signal Transduction
Zdroj:	Journal of food biochemistryREFERENCES. 46(12)
ISSN:	1745-4514
Popis:	This study examined the antidiabetic efficacy of popular fermented soybean foods (FSF) of Northeast (NE) India. Results showed that among different FSF, aqueous extract of Hawaijar (AEH), a traditional FSF of Manipur, NE India, significantly augmented glucose utilization in cultured myotubes treated with high glucose (HG, 25 mM). Furthermore, AEH also upregulated glucose uptake, glucose-6-phosphate level, and phopho-PI3K/phospho-AKT/phospho-AMPK/GLUT4 protein expression in HG-treated myotubes. In vivo studies demonstrated that AEH supplementation (50, 100, or 200 mg/kg body weight/day, oral gavaging, 16 weeks) reduced body weight, fasting blood glucose, glycated hemoglobin, insulin resistance, and glucose intolerance in rats fed with high-fat diet (HFD). AEH supplementation stimulated phopho-PI3K/phospho-AKT/phospho-AMPK/GLUT4 signaling cascades involved in glucose metabolism of muscle tissues in diabetic rats. Chemical profiling of AEH (SDS-PAGE, immunoblotting, and HRMS) suggests the possible role of bioactive proteins/peptides and isoflavones underlying the antihyperglycemic potential AEH. Results from this study will be helpful for developing food-based prophylactics/therapeutics in managing hyperglycemia. PRACTICAL APPLICATIONS: Fermented soybean foods are gaining acceptance due to multiple health benefits. This study for the first time reports the antidiabetic potential of Hawaijar, an indigenous fermented soybean food of North-East India. Higher abundance of bioactive compounds (isoflavones and proteins/peptides) in Hawaijar may be responsible for the alleviation of impaired glucose metabolism associated with diabetes. The findings may be helpful for the development of a novel therapeutic to achieve better control of hyperglycemia and improve the lives of the patient population with diabetes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79792df5acbbed49213dc9d51501d4ee https://pubmed.ncbi.nlm.nih.gov/36069251 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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