Dose-dependent regulation of target gene expression and cell proliferation by c-Myc levels
| Autor: | Marino Schuhmacher, Dirk Eick |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
titration
Leucine zipper Gene Expression Biology Biochemistry Proto-Oncogene Mas Cell Line Proto-Oncogene Proteins c-myc Transactivation transactivation Gene expression Genetics Humans RNA Messenger Gene Transcription factor transcription factor Cell Proliferation Cell Size Cell growth Tetracycline Molecular biology Cell culture gene activity S Phase Cell Cycle Checkpoints Biotechnology Research Paper |
| Zdroj: | Transcription |
| ISSN: | 2154-1272 2154-1264 |
| Popis: | The proto-oncogene c-myc encodes a basic helix-loop-helix leucine zipper transcription factor (c-Myc). c-Myc plays a crucial role in cell growth and proliferation. Here, we examined how expression of c-Myc target genes and cell proliferation depend on variation of c-Myc protein levels. We show that proliferation rates, the number of cells in S-phase, and cell size increased in a dose-dependent manner in response to increasing c-Myc levels. Likewise, the mRNA levels of c-Myc responsive genes steadily increased with rising c-Myc levels. Strikingly, steady-state mRNA levels of c-Myc target genes did not saturate even at highest c-Myc concentrations. These characteristics predestine c-Myc levels as a cellular rheostat for the control and fine-tuning of cell proliferation and growth rates. |
| Databáze: | OpenAIRE |
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