Comparative transcriptome analysis of flower bud transition and functional characterization of EjAGL17 involved in regulating floral initiation in loquat

Autor: Feng Zhan, Qigao Guo, Di Wu, Shuming Wang, Guolu Liang, Min Shi, Danlong Jing, Baogui Xue, Qiao He, Yan Xia
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Gene Expression
Plant Science
01 natural sciences
Biochemistry
Transcriptome
Cell Signaling
Plant Growth Regulators
Arabidopsis thaliana
MYB
Phylogeny
Plant Proteins
Multidisciplinary
biology
Bud
Agamous
Plant Anatomy
Eukaryota
food and beverages
Genomics
Plants
Signaling Cascades
Cell biology
Ethylene Signaling Cascade
Protein Transport
Experimental Organism Systems
Medicine
Buds
Transcriptome Analysis
Research Article
Signal Transduction
Arabidopsis Thaliana
Science
DNA transcription
Eriobotrya
Brassica
Flowers
Research and Analysis Methods
03 medical and health sciences
Model Organisms
Plant and Algal Models
DNA-binding proteins
Genetics
Gene Regulation
Amino Acid Sequence
Biology and life sciences
Gene Expression Profiling
fungi
Organisms
Proteins
Computational Biology
Cell Biology
biology.organism_classification
Genome Analysis
WRKY protein domain
Regulatory Proteins
030104 developmental biology
Animal Studies
Ectopic expression
010606 plant biology & botany
Transcription Factors
Zdroj: PLoS ONE, Vol 15, Iss 10, p e0239382 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Floral initiation plays a critical role for reproductive success in plants, especially fruit trees. However, little information is known on the mechanism of the initiation in loquat (Eriobotrya japonica Lindl.). Here, we used transcriptomic, expression and functional analysis to investigate the candidate genes in floral initiation in loquat. Comparative transcriptome analysis showed differentially expressed genes (DEGs) were mainly enriched in the metabolic pathways of plant hormone signal transduction. The DEGs were mainly involved in the gibberellin, auxin, cytokinin, abscisic acid, salicylic acid and ethylene signaling pathways. Meanwhile, some transcription factors, including MADS-box (MCM1, AGAMOUS, DEFICIENS and SRF), MYB (Myeloblastosis), TCP (TEOSINTE BRANCHED 1, CYCLOIDEA and PCF1), WOX (WUSCHEL-related homeobox) and WRKY (WRKY DNA-binding protein), were significantly differentially expressed. Among these key DEGs, we confirmed that an AGL17 ortholog EjAGL17 was significantly upregulated at the flower bud transition stage. Phylogenetic tree analysis revealed that EjAGL17 was grouped into an AGL17 clade of MADS-box transcription factors. Protein sequence alignment showed that EjAGL17 included a distinctive C-terminal domain. Subcellular localization of EjAGL17 was found only in the nucleus. Expression levels of EjAGL17 reached the highest at the development stage of flower bud transition. Moreover, ectopic expression of EjAGL17 in Arabidopsis significantly exhibited early flowering. Our study provides abundant resources of candidate genes for studying the mechanisms underlying the floral initiation in loquat and other Rosaceae species.
Databáze: OpenAIRE
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