Protective effects of genistein on cytochrome P-450 and vitellogenin expression in liver of zebrafish after PCB-126 exposure
| Autor: | Eugenia Rita Lauriano, Patrizia Licata, Francesco Fazio, Margherita Calò, Giuseppe Piccione |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Environmental Engineering 010504 meteorology & atmospheric sciences Cytochrome Genistein Model system PCB-126Immunoreactivity Danio rerio CYP1A1 Vitellogenin 010501 environmental sciences 01 natural sciences Vitellogenins chemistry.chemical_compound Vitellogenin Cytochrome P-450 Enzyme System Internal medicine medicine Animals Environmental Chemistry heterocyclic compounds Waste Management and Disposal Zebrafish Volume concentration 0105 earth and related environmental sciences biology Chemistry organic chemicals food and beverages Zebrafish Proteins biology.organism_classification Polychlorinated Biphenyls Pollution stomatognathic diseases Endocrinology Liver embryonic structures biology.protein Immunohistochemistry Water Pollutants Chemical |
| Popis: | The objective of the research is to study the action of Vitellogenin and P-4501A1 following coexposure at different times to genistein and PCB-126 using zebrafish as a model system. Polychlorinated biphenyls are ubiquitous substances in environment. The genistein is a phytoestrogen extracted from soybeans and it's contained in food for humans and animals. For this study, 200 adult zebrafish were used. Our findings show a marked immunoreactivity of Vtg at 12h in liver than the control with only PCB-126. Regarding effects of PCB-126 on Vtg after pretreatment with genistein in fishes, the immunohistochemistry results show a minor increase at 12h. After 24h the immunoreactivity is lower than 12h and then slightly increased at 72h with only PCB-126 and PCB-126 and genistein together. CYP1A1 progressively increases from 12h to 72h in all groups with minor immunoreactivity when we treated fish with genistein and PCB-126. We show a reduction in the estrogenic effect when the fishes were treated with genistein and PCB-126 together at 12h than the group treated with only PCB-126. Moreover, low concentrations of genistein decrease the marked P450 expression induced by PCB-126. This shows that genistein decreases the expression of P450 target genes mediated by AhR. |
| Databáze: | OpenAIRE |
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