| Autor: |
Ce-Jie, Lan, Ai-Juan, Sun, Qin-Mei, Liu, Qing-Ming, Shi, Xiao-Xia, Guo, Dan-Xing, Heng-Duan, Zhang, Yan-de, Dong, Chun-Xiao, Li, Tong-Yan, Zhao |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Acta Tropica. 236:106698 |
| ISSN: |
0001-706X |
| DOI: |
10.1016/j.actatropica.2022.106698 |
| Popis: |
Dengue virus type Ⅱ (DENV2) is a primary serotype responsible for the dengue fever epidemic, and Aedes aegypti is the main DENV2 vector. Understanding the Aedes aegypti immune mechanism against DENV2 is the basis for research on immune blockade in mosquitoes. Some preliminary studies lack validation in the literature, so this study was performed to further study and validate the potential target genes to provide a further basis for screening key target genes. We screened 51 genes possibly related to Aedes aegypti infection and immunity from the literature for further verification. First, bioinformatic methods such as GO, KEGG and PPI analysis were used, and then RT-qPCR was used to detect the changes in mRNA expression in the midguts and salivary glands of Aedes aegypti infected with DENV2.Bioinformatic analysis showed that mostly genes of the glucose metabolism pathway and myoprotein were influenced. In salivary glands, the Gst (x |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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