1,2-Diacylglycerols do not potentiate the action of phospholipases A2 and C in human platelets
| Autor: | Barry R. Ganong, Eduardo G. Lapetina, Robert M. Bell, Steve P. Watson |
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| Rok vydání: | 1984 |
| Předmět: |
Blood Platelets
Biophysics In Vitro Techniques Phospholipase Biology Biochemistry Phospholipases A Glycerides Diglycerides chemistry.chemical_compound Hydrolysis Humans Platelet Inositol Phosphorylation Molecular Biology Phospholipase A Blood Proteins Cell Biology Phosphatidic acid Phospholipases A2 chemistry Phospholipases Type C Phospholipases Arachidonic acid |
| Zdroj: | Biochemical and Biophysical Research Communications. 121:386-391 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(84)90734-4 |
| Popis: | 1,2-Diacylglycerol has recently been reported to potentiate the ability of phospholipases A and C to hydrolyze phospholipids in a cellfree system. The present study has been undertaken to investigate whether 1,2-diacylglycerol can also perform this function in intact cells using the platelet as a test system. Exogenous 1-oleoyl-2-acetyl-glycerol (OAG) and 1,2-didecanoylglycerol, at concentrations sufficient to produce maximal phosphorylation of a 40,000 dalton protein, caused no significant formation of [ 3 H]inositol phosphates and [ 32 P]phosphatidic acid (products of phospholipase C activation) or [ 14 C]arachidonic acid metabolites and lysophosphatidyl[ 3 H]inositol (products of phospholipase A 2 activation). These data therefore imply that 1,2-diacylglycerols do not potentiate the actions of phospholipases A 2 and C in intact platelets at concentrations that are physiologically relevant. |
| Databáze: | OpenAIRE |
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