Presence of Human Papillomavirus and Epstein-Barr Virus, but Absence of Merkel Cell Polyomavirus, in Head and Neck Cancer of Non-Smokers and Non-Drinkers
| Autor: | Stefan M. Willems, Bernd Kremer, Ernst-Jan M. Speel, Frans J. Mulder, Faisal Klufah, Famke M E Janssen, Farzaneh Farshadpour, Mari F.C.M. van den Hout, Axel zur Hausen, Remco de Bree |
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| Přispěvatelé: | RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, KNO, MUMC+: MA AIOS Keel Neus Oorheelkunde (9), RS: GROW - R2 - Basic and Translational Cancer Biology, Pathologie, MUMC+: DA Klinische Pathologie (5), MUMC+: DA Pat Pathologie (9), MUMC+: MA Keel Neus Oorheelkunde (3), MUMC+: MA Keel Neus Oorheelkunde (9), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Damage and Repair in Cancer Development and Cancer Treatment (DARE) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cancer Research non-drinkers polyomavirus Merkel cell polyomavirus In situ hybridization medicine.disease_cause lcsh:RC254-282 non-smokers Virus Epstein–Barr virus 03 medical and health sciences 0302 clinical medicine cell cycle protein medicine human papillomavirus Original Research Tissue microarray biology business.industry Head and neck cancer lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease biology.organism_classification Head and neck squamous-cell carcinoma stomatognathic diseases 030104 developmental biology Oncology 030220 oncology & carcinogenesis Barr virus Cancer research Immunohistochemistry epstein– head and neck cancer in situ hybridization business |
| Zdroj: | Frontiers in Oncology Frontiers in Oncology, Vol 10 (2021) Frontiers in Oncology, 10:560434. Frontiers Media S.A. Frontiers in Oncology, 10:560434, 1-12. Frontiers Media SA |
| ISSN: | 2234-943X |
| Popis: | ObjectiveDetermine the presence and prognostic value of human papillomavirus (HPV), Epstein-Barr virus (EBV), Merkel cell polyomavirus (MCPyV), and cell cycle proteins in head and neck squamous cell carcinoma (HNSCC) of non-smokers and non-drinkers (NSND).MethodsClinical characteristics and tumors of 119 NSND with HNSCC were retrospectively collected and analyzed on tissue microarrays. RNAscope in situ hybridization (ISH) was used to screen for the presence of HPV and MCPyV mRNA. Immunohistochemistry was performed for expression of p16 as surrogate marker for HPV, Large T-antigen for MCPyV, and cell cycle proteins p53 and pRb. Positive virus results were confirmed with polymerase chain reaction. For EBV, EBV encoded RNA ISH was performed. Differences in 5-year survival between virus positive and negative tumors were determined by log rank analysis.ResultsAll oropharyngeal tumors (OPSCC) (n = 10) were HPV-positive, in addition to one oral (OSCC) and one nasopharyngeal tumor (NPSCC). The other three NPSCC were EBV-positive. MCPyV was not detected. Patients with HPV or EBV positive tumors did not have a significantly better 5-year disease free or overall survival. Over 70% of virus negative OSCC showed mutant-type p53 expression.ConclusionIn this cohort, all OPSCC and NPSCC showed HPV or EBV presence. Besides one OSCC, all other oral (n = 94), hypopharyngeal (n = 1), and laryngeal (n = 9) tumors were HPV, EBV, and MCPyV negative. This argues against a central role of these viruses in the ethiopathogenesis of tumors outside the oro- and nasopharynx in NSND. So, for the majority of NSND with virus negative OSCC, more research is needed to understand the carcinogenic mechanisms in order to consider targeted therapeutic options. |
| Databáze: | OpenAIRE |
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