Long non‐coding RNA MEG3 promotes autophagy and apoptosis of nasopharyngeal carcinoma cells via PTEN up‐regulation by binding to microRNA‐21
Autor: | Baotao Lv, Xiaoli Liu, Liqiang Lin |
---|---|
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
autophagy Carcinogenesis maternally expressed gene 3 Down-Regulation Mice Nude Apoptosis Biology Models Biological microRNA‐21 03 medical and health sciences 0302 clinical medicine Cell Line Tumor microRNA medicine Animals Humans PTEN MEG3 Mice Inbred BALB C Reporter gene Nasopharyngeal Carcinoma Base Sequence Autophagy PTEN Phosphohydrolase Original Articles Cell Biology Transfection Prognosis medicine.disease Long non-coding RNA Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Nasopharyngeal carcinoma 030220 oncology & carcinogenesis biology.protein Cancer research Molecular Medicine Original Article RNA Long Noncoding phosphatase and tensin homologue Protein Binding |
Zdroj: | Journal of Cellular and Molecular Medicine |
ISSN: | 1582-4934 1582-1838 |
Popis: | Long non‐coding RNAs (lncRNAs) have been highlighted as attractive markers for diagnosis and prognosis as well as new therapeutic targets in multiple cancers, including nasopharyngeal carcinoma (NPC). Here, we attempted to investigate the underlying regulatory role of the lncRNA maternally expressed gene 3 (MEG3) in NPC development. As determined by RT‐qPCR, MEG3 expression was down‐regulated in NPC cells. Online RNA crosstalk analysis predicted the binding of miR‐21 to MEG3 and PTEN, respectively. MEG3 was validated to bind to miR‐21 while PTEN was identified as a target of miR‐21 by dual‐luciferase reporter gene assay. Exogenous transfection was done to change the levels of MEG3, miR‐21 and PTEN in HK‐1 cells to investigate their effects on the autophagy and apoptosis of NPC cells. The results suggested that MEG3 overexpression in HK‐1 cells up‐regulated PTEN and down‐regulated miR‐21, by which MEG3 further inhibited autophagy and apoptosis ability of NPC cells. The tumour formation ability was tested after injecting the HK‐1 cells into nude, mice and tumour growth was monitored. Consistently, MEG3 overexpression inhibited the tumour formation in vivo. Collectively, MEG3 promotes the autophagy and apoptosis of NPC cells via enhancing PTEN expression by binding to miR‐21. |
Databáze: | OpenAIRE |
Externí odkaz: |