Detection of DNA of genetically modified maize by a silicon nanowire field-effect transistor
| Autor: | Phu Duy Tran, Mau Chien Dang, Cees J M van Rijn, Van Binh Pham, Xuan Thanh Tung Pham, Thi Thanh Tuyen Le, Thanh Chien Nguyen, Van Quoc Nguyen, Ngoc Thuy Duong Dang, Duy Hien Tong |
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| Přispěvatelé: | Pham, Van Binh, Pham, Xuan Thanh Tung, Dang, Ngoc Thuy Duong, Le, Thi Thanh Tuyen, Tran, Phu Duy, Nguyen, Thanh Chien, Nguyen, Van Quoc, Dang, Mau Chien, Van Rijn, Cees JM, Tong, Duy Hien |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
DNA detection
Genetically modified maize Materials science Oligonucleotide Transistor Nanotechnology Industrial and Manufacturing Engineering DNA sequencing law.invention chemistry.chemical_compound chemistry law Etching (microfabrication) General Materials Science Field-effect transistor silicon nanowire nanosensor Electrical and Electronic Engineering deposition and etching under angles Biosensor surface modification DNA |
| Popis: | A silicon nanowire field-effect transistor based sensor (SiNW-FET) has been proved to be the most sensitive and powerful device for bio-detection applications. In this paper, SiNWs were first fabricated by using our recently developed deposition and etching under angle technique (DEA), then used to build up the complete SiNW device based biosensor. The fabricated SiNW biosensor was used to detect DNA of genetically modified maize. As the DNA of the genetically modified maize has particular DNA sequences of 35S promoter, we therefore designed 21 mer DNA oligonucleotides, which are used as a receptor to capture the transferred DNA of maize. In our work, the SiNW biosensor could detect DNA of genetically modified maize with concentrations down to about 200 pM. |
| Databáze: | OpenAIRE |
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