Effect of quercetin on oxidative nuclear and mitochondrial DNA damage
| Autor: | Cinzia Calcabrini, Lucia Potenza, Roberta De Bellis, Umberto Mancini, Luigi Cucchiarini, Marina Dachà |
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| Přispěvatelé: | Potenza, Lucia, Calcabrini, Cinzia, De Bellis, Roberta, Mancini, Umberto, Cucchiarini, Luigi, Dach, Marina |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Keratinocytes
Mitochondrial DNA DNA damage Cell Survival Ultraviolet Rays Clinical Biochemistry Oxidative phosphorylation Biology DNA Mitochondrial Polymerase Chain Reaction Biochemistry Antioxidants law.invention Mixed Function Oxygenases chemistry.chemical_compound law Oxidative damage Humans heterocyclic compounds Chromans Polymerase chain reaction Cells Cultured Cell Nucleus NCTC 2544 Nuclear DNA General Medicine Hydrogen Peroxide Molecular biology Mitochondria Real-time polymerase chain reaction chemistry Molecular Medicine Quercetin Oxidation-Reduction DNA DNA Damage |
| Popis: | Quercetin is a well-investigated antioxidant known to protect cells against oxidative nuclear DNA damage. There is no knowledge regarding its effect on oxidative mitochondrial DNA damage. In this study we investigated the effect of quercetin on oxidatively-injured DNA. Cell-free and cell studies were performed. Cell-free analyses carried out on plasmidic DNA showed that quercetin protects from all oxidative challenges used. Cellular studies were carried out on NCTC 2544 cells which were insulted with hydrogen peroxide and UVC radiations. Nuclear and mitochondrial DNAs were analysed by measuring DNA damage with a quantitative polymerase chain reaction. Quercetin supplementation showed significant genoprotective activity on mitochondrial DNA when hydroperoxide was used. The evidence of the protection afforded by quercetin suggests that this flavonoid may play an important role on mitochondrial genome stability. © 2008 IUBMB/IOS Press and the authors. All rights reserved. |
| Databáze: | OpenAIRE |
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