In vitro Evaluation of CMV Specific CD8+T Cells Function in CMV+ Colorectal Cancer Patients Compared to Healthy Controls
| Autor: | Yadollah Shakiba, Ali Abbasian, Seyed Morteza Tabatabaei Yazdi, Fatemeh Pak, Maryam Nourizadeh, Parviz Kokhaei, Mohammad Sadegh Fazeli, Mona Shaker Ardakani |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Human cytomegalovirus T cell medicine.medical_treatment Epitopes T-Lymphocyte Cytomegalovirus Interferon gamma (IFN-γ) lcsh:Medicine T-Cell Antigen Receptor Specificity CD8-Positive T-Lymphocytes Antibodies Viral Epitope Immunophenotyping CD107a medicine Humans Immunology and Allergy Cytotoxic T cell Antigens Viral Cells Cultured Aged Aged 80 and over Solid tumor business.industry lcsh:R Immunotherapy Middle Aged medicine.disease Real-time polymerase chain reaction medicine.anatomical_structure Case-Control Studies Immunoglobulin G Cytomegalovirus Infections DNA Viral Cancer research Cytokines Female Tumor necrosis factor alpha Colorectal Neoplasms business Biomarkers CD8 |
| Zdroj: | Iranian Journal of Allergy, Asthma and Immunology, Vol 18, Iss 4 (2019) |
| ISSN: | 1735-5249 1735-1502 |
| Popis: | The oncogenic role of human cytomegalovirus (HCMV) has been recently shown in different cancers like colorectal cancer (CRC). According to the recent immunotherapy approach to target the CMV-expressing tumor cells, we investigated the CMV peptide-stimulated CD8+T cells functions in CRC patients compared to healthy individuals. All sixteen patients and seven controls were CMV seropositive. Blood samples were obtained from patients without chemotherapy and radiotherapy before surgery. Cytotoxic CD8+ T cells were generated using 14-day culture of PBMCs in the presences of CMV peptide epitopes and rhIL-2. In addition to the supernatant evaluations for TNF-α and IFN-γ, the functionality of CD8+ T cells was examined by detecting CD107a and intracellular IFN-γ using flow cytometry. CMV DNA was detected in tissues by Real Time PCR. CMV DNA was found in 31% of tumor tissues, while it was not seen in the adjacent non-tumor tissues. There was a close association between CMV in tumor tissue and tumor grade. Surface expression of CD107a and intracellular IFN-γ in CMV-stimulated CD8+T cells and the level of IFN-γ production in patient and control groups increased significantly after culture. The number of functions increased in patients (p |
| Databáze: | OpenAIRE |
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