FAHFAs Regulate the Proliferation of C2C12 Myoblasts and Induce a Shift toward a More Oxidative Phenotype in Mouse Skeletal Muscle

Autor: Charles Coudray, François Casas, Sylvie Gaillet, Gilles Fouret, Bénédicte Goustard, Thierry Durand, Christine Feillet-Coudray, Laurence Pessemesse, Melha Benlebna, Laurence Balas, Béatrice Bonafos, Laura Pavlin
Přispěvatelé: Dynamique Musculaire et Métabolisme (DMEM), Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), PhD program of the Algerian Ministry of Higher Education and Scientific Research, French Lipid Nutrition Group, National Research Institute for Agriculture, Food, and Environment (INRAE)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Myoblast proliferation
[SDV]Life Sciences [q-bio]
medicine.disease_cause
mitochondrial activity
Receptors
G-Protein-Coupled
lcsh:Chemistry
Myoblasts
0302 clinical medicine
FAHFAs
Myosin
Myocyte
lcsh:QH301-705.5
Spectroscopy
chemistry.chemical_classification
0303 health sciences
Fatty Acids
Cell Differentiation
Esters
General Medicine
musculoskeletal system
Mitochondria
Computer Science Applications
Phenotype
medicine.anatomical_structure
C2C12
Oxidation-Reduction
tissues
medicine.medical_specialty
mice
Oxidative phosphorylation
Article
Catalysis
Cell Line
Electron Transport
Electron Transport Complex IV
Inorganic Chemistry
03 medical and health sciences
Internal medicine
medicine
Animals
RNA
Messenger
Physical and Theoretical Chemistry
skeletal muscle
Muscle
Skeletal
Molecular Biology
Cell Proliferation
030304 developmental biology
Organic Chemistry
Skeletal muscle
Fatty acid
Mice
Inbred C57BL
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
chemistry
030217 neurology & neurosurgery
Oxidative stress
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 23
International Journal of Molecular Sciences, MDPI, 2020, 21 (23), pp.9046. ⟨10.3390/ijms21239046⟩
International Journal of Molecular Sciences, Vol 21, Iss 9046, p 9046 (2020)
ISSN: 1422-0067
1661-6596
DOI: 10.3390/ijms21239046
Popis: Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are endogenous lipids reported to have antidiabetic and anti-inflammatory effects. Since skeletal muscle is a major target for insulin, the aim of this study is to explore for the first time the influence of several FAHFAs in C2C12 myoblasts and in skeletal muscle phenotype in mice. Here, we show that eleven FAHFAs belonging to different families inhibit C2C12 myoblast proliferation. In addition, all FAHFAs decreased mitochondrial cytochrome c oxidase activity without affecting reactive oxygen species production and the mitochondrial network. During C2C12 myoblasts differentiation, we found that two of the most active lipids, 9-PAHPA and 9-OAHPA, did not significantly affect the fusion index and the expression of myosin heavy chains. However, we found that three months&rsquo
intake of 9-PAHPA or 9-OAHPA in mice increased the expression of more oxidative myosin in skeletal muscle without affecting skeletal muscle mass, number, and mean fiber area, mitochondrial activity, and oxidative stress parameters. In conclusion, our study indicated that the eleven FAHFAs tested decreased the proliferation rate of C2C12 myoblasts, probably through the inhibition of mitochondrial activity. In addition, we found that 9-PAHPA or 9-OAHPA supplementation in mice induced a switch toward a more oxidative contractile phenotype of skeletal muscle. These data suggest that the increase in insulin sensitivity previously described for these two FAHFAs is of muscular origin.
Databáze: OpenAIRE
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