Carbon–protein covalent conjugates in non-instrumental immunodiagnostic systems
| Autor: | Mikhail Rayev, Konstantin Shmagel |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Immunology Covalent binding chemistry.chemical_element HIV Infections Nerve Tissue Proteins HIV Antibodies Immunologic Tests Chorionic Gonadotropin Carbon particle Colloid Humans Immunology and Allergy Colloids Chromatography Chemistry Combinatorial chemistry Carbon Covalent bond Reagent HIV-2 HIV-1 Female Streptavidin Conjugate |
| Zdroj: | Journal of Immunological Methods. 336:9-15 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/j.jim.2008.03.005 |
| Popis: | An original technique for obtaining stable conjugates using colloid carbon particles as indicator labels has been developed. The reliability and stability of the diagnostic reagents obtained is provided by covalent binding of various affine compounds on the surface of carbon particles. The stability of the reagents has been studied under various storage conditions for 3-10 years. It was shown that even when storage conditions were outside the optimal range, some conjugates preserved their analytical characteristics for 10 years. A number of systems for analytical detection of various ligands have been designed based on the carbon-protein conjugates synthesized. The major characteristics of these systems are their high sensitivity and specificity, reliability, and reproducibility, simplicity in operation, and quick results. |
| Databáze: | OpenAIRE |
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