Denaturing for Nanoarchitectonics: Local and Periodic UV-Laser Photodeactivation of Protein Biolayers to Create Functional Patterns for Biosensing

Autor: Augusto Juste-Dolz, Martina Delgado-Pinar, Miquel Avella-Oliver, Estrella Fernández, Jose Luís Cruz, Miguel V. Andrés, Ángel Maquieira
Rok vydání: 2022
Předmět:
Zdroj: Juste-Dolz, Augusto Delgado-Pinar, Martina Avellà-Oliver, Miquel Fernández, Estrella Cruz Muñoz, José Luis Andrés, Miguel V. Maquieira, Ángel 2022 Denaturing for nanoarchitectonics: local and periodic UV-laser photodeactivation of protein biolayers to create functional patterns for biosensing Acs Applied Materials & Interfaces 14 36 41640 41648
RODERIC. Repositorio Institucional de la Universitat de Valéncia
instname
ISSN: 1944-8252
1944-8244
Popis: [EN] The nanostructuration of biolayers has become a paradigm for exploiting nanoscopic light-matter phenomena for biosensing, among other biomedical purposes. In this work, we present a photopatterning method to create periodic structures of biomacromolecules based on a local and periodic mild denaturation of protein biolayers mediated by UV-laser irradiation. These nanostructures are constituted by a periodic modulation of the protein activity, so they are free of topographic and compositional changes along the pattern. Herein, we introduce the approach, explore the patterning parameters, characterize the resulting structures, and assess their overall homogeneity. This UV-based patterning principle has proven to be an easy, cost-effective, and fast way to fabricate large areas of homogeneous one-dimensional protein patterns (2 min, 15 x 1.2 mm, relative standard deviation ? 16%). This work also investigates the implementation of these protein patterns as transducers for diffractive biosensing. Using a model immunoassay, these patterns have demonstrated negligible signal contributions from non-specific bindings and comparable experimental limits of detection in buffer media and in human serum (53 and 36 ng & BULL;mL-1 of unlabeled IgG, respectively).
This work was financially supported by the Ministerio de Ciencia e Innovacion/Agencia Estatal de Investigacion (MCIN/AEI/10.13039/501100011033) , co-funded by the European Union "ERDF A way of making Europe", under grants PID2019-110713RB-I00 and PDI2019-104276RB-I00, and Generalitat Valenciana (PROMETEO/2020/094 and PROMETEO/2019/048) . A.J.-D. acknowledges the FPI-UPV 2017 grant program. The authors thank Angel Lopez Munoz for the construction of the scanning system.
Databáze: OpenAIRE
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