Attachment of flagellin enhances the immunostimulatory activity of a hemagglutinin-ferritin nano-cage
| Autor: | Emerson B. Lee, Hyun-Jin Kim, Young-Jin Kim, Hayyoung Lee, Hyung-Min Jeon, Jie-Oh Lee, Young Sil Kim, Doo-Jin Kim, Sang M. Park, Chang-Ung Kim, Geunyoung Cho |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Salmonella typhimurium
medicine.medical_treatment T cell Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Hemagglutinin Glycoproteins Influenza Virus 02 engineering and technology Microbiology Cell Line 03 medical and health sciences Mice Immune system Adjuvants Immunologic medicine Animals Humans General Materials Science Secretion 030304 developmental biology 0303 health sciences Immunity Cellular Mice Inbred BALB C biology Chemistry Hemagglutinin 021001 nanoscience & nanotechnology Immunity Humoral Nanostructures Ferritin medicine.anatomical_structure Influenza A virus Ferritins biology.protein bacteria Molecular Medicine Female Antibody 0210 nano-technology Adjuvant Flagellin |
| Zdroj: | Nanomedicine : nanotechnology, biology, and medicine. 17 |
| ISSN: | 1549-9642 |
| Popis: | Hemagglutinin (HA) displayed on a ferritin nano-cage has been shown to be effective in generating a potent immune response against a broad range of influenza infections. Here, we showed that conjugation of flagellin together with HA to the exterior surface of the ferritin cage greatly enhanced not only the humoral immune response in mice but also antigen-specific T cell responses that include Th1 cytokine secretion. The effect of flagellin remained essentially unchanged when the molar ratio of flagellin to HA was reduced from 1:1 to 1:3. Injection of the ferritin-HA-flagellin cage provided protection against lethal virus challenge in mice. We used a small immunoglobulin fragment VL12.3 as a convenient method for attaching HA and flagellin to the ferritin cage. This attachment method can be used for rapid screening of a variety of protein cages and nano-assemblies to identify the most suitable carrier and adjuvant proteins for the target antigen. |
| Databáze: | OpenAIRE |
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