Treatment with spermidine alleviates the effects of concomitantly applied cold stress by modulating Ca2+, pH and ROS homeostasis, actin filament organization and cell wall deposition in pollen tubes of Camellia sinensis
Autor: | Giampiero Cai, Stefano Del Duca, Aslıhan Çetinbaş-Genç |
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Přispěvatelé: | Cetinbas-Genc A., Cai G., Del Duca S. |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Actin cytoskeleton Cell wall Cold stress Pollen tube Polyamines Spermidine Polyamine Physiology Actin filament organization Pollen Tube Plant Science medicine.disease_cause 01 natural sciences Actin cytoskeleton organization Camellia sinensi 03 medical and health sciences chemistry.chemical_compound Pollen Homeostasi otorhinolaryngologic diseases Genetics medicine Cold-Shock Response Callose food and beverages Hydrogen-Ion Concentration 030104 developmental biology chemistry Germination Biophysics Cold stre Reactive Oxygen Specie 010606 plant biology & botany |
Zdroj: | Plant Physiology and Biochemistry. 156:578-590 |
ISSN: | 0981-9428 |
Popis: | The aim of the current study was to examine the effect of spermidine treatment concomitant with cold stress on the elongation of Camellia sinensis pollen tube. When exogenous spermidine (0.05 mM) was applied concomitantly with cold stress, pollen germination rate and pollen tube length were significantly increased in comparison with cold stressed pollen tubes. In addition, spermidine treatment concomitantly with cold stress reduced pollen tube abnormalities induced by cold stress. Besides, cold-induced disorganizations of actin filaments were ameliorated after spermidine treatment along with cold stress because anisotropy levels of actin filaments in shank and apex of pollen tubes decreased. Changes in cold-induced callose distribution in the pollen tube cell wall were partially recovered after spermidine/cold stress treatment. Other cold-induced effects (decrease in Ca2+ content, reduction of pH gradient, accumulation of ROS) were reverted to adequate levels after spermidine treatment in conjunction with cold stress, indicating that pollen tubes are able to cope with stress. Thus, spermidine treatment reorganized the growth pattern of pollen tubes by modulating Ca2+ and ROS homeostasis, actin cytoskeleton organization, and cell wall deposition in Camellia sinensis pollen tubes under cold stress. |
Databáze: | OpenAIRE |
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