Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR
| Autor: | Daniel E. Wilson, Susanne Schoch, Edwin R. Chapman, Victor J. DePiero, Huan Bao, Loren L. Looger, Ariana N. Tkachuk, David A. DiGregorio, Abbas Kazemipour, Justin P. Little, Benjamin Scholl, Johannes Alexander Müller, Kaspar Podgorski, Bart G. Borghuis, David Fitzpatrick, Nelson Rebola, Dirk Dietrich, Jonathan S. Marvin, Samuel S.-H. Wang, Adam W. Hantman, Edward Cai, Francisco José Urra Quiroz |
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| Přispěvatelé: | Howard Hughes Medical Institute (HHMI), Max Planck Florida Institute for Neuroscience (MPFI), Max-Planck-Gesellschaft, Rheinische Friedrich-Wilhelms-Universität Bonn, Dynamique des synapses et des circuits neuronaux - Synapse and circuit dynamics, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), University of Wisconsin-Madison, Princeton University, University of Louisville |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Dendritic spine genetic structures Cyan [SDV]Life Sciences [q-bio] Green Fluorescent Proteins Color Glutamic Acid Biochemistry Article Retina 03 medical and health sciences 0302 clinical medicine In vivo Microscopy Animals Chromatic scale Molecular Biology Fluorescent Dyes Visual Cortex Neurons Fluorescent reporter Chemistry Glutamate receptor Ferrets Cell Biology Fluorescence Mice Inbred C57BL 030104 developmental biology Microscopy Fluorescence Biophysics Female sense organs 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | Nature methods Nature Methods Nature Methods, 2018, 15 (11), pp.936-939. ⟨10.1038/s41592-018-0171-3⟩ |
| ISSN: | 1548-7105 1548-7091 |
| DOI: | 10.1038/s41592-018-0171-3⟩ |
| Popis: | International audience; Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates. |
| Databáze: | OpenAIRE |
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