Expression and localization of adiponectin and its receptors (AdipoR1 and AdipoR2) in the hypothalamic-pituitary-ovarian axis of laying hens
| Autor: | Na Zhang, Yan Fengbin, Chong Li, Kui Li, Xiaojun Liu, Yadong Tian, Qi Li, Jing Li, Yijie Li, Hong Li, Xiangtao Kang, Yuanfang Li |
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| Rok vydání: | 2020 |
| Předmět: |
endocrine system
medicine.medical_specialty Adipose tissue Hypothalamic–pituitary–gonadal axis Biology Energy homeostasis 03 medical and health sciences 0302 clinical medicine Food Animals Internal medicine medicine Animals Theca externa Small Animals Receptor 030219 obstetrics & reproductive medicine Adiponectin Equine Ovary 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science Endocrinology Hypothalamus Pituitary Gland Animal Science and Zoology Female Receptors Adiponectin Chickens hormones hormone substitutes and hormone antagonists Immunostaining |
| Zdroj: | Theriogenology. 159 |
| ISSN: | 1879-3231 |
| Popis: | Adiponectin is a hormone secreted by adipose tissue that is involved in the regulation of energy homeostasis and reproduction. In this study, the expression levels of adiponectin and its receptors in the hypothalamic-pituitary-ovarian (HPO) axis of laying hens were investigated using quantitative real-time PCR (qRT-PCR) and Western blotting, and the localization of these proteins was explored using immunohistochemistry. The morphological relationships between adiponectin receptors and gonadotropin-releasing hormone (GnRH) neurons were analyzed using double immunofluorescence labeling. The results showed that adiponectin mRNA and protein were widely expressed in all tissues involved in the HPO axis in laying hens, with especially high expression in the hypothalamus. Both AdipoR1 and AdipoR2 were more highly expressed in the pituitary than in other tissues and exhibited similar mRNA and protein expression patterns. The immunohistochemistry results showed that adiponectin and AdipoR2 were localized in the major hypothalamic nuclei that regulate food intake and energy balance (i.e., the lateral hypothalamic area (LHA), infundibular nucleus (IN), dorsomedial nucleus (DMN), and paraventricular nucleus (PVN)). Immunostaining revealed that adiponectin and its receptors were also localized in the cytoplasm of cells in the adenohypophysis. In the ovaries, adiponectin was localized in the granulosa layer, in the theca externa of follicles and in basal cells, while AdipoR1 and AdipoR2 were localized in basal cells. In the double immunofluorescence labeling experiment, AdipoR1 and AdipoR2 were localized in GnRH neurons in the IN and DMN. These results suggest that adiponectin and its receptors may play major roles in the endocrine network, which integrates energy balance and reproduction. |
| Databáze: | OpenAIRE |
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