Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from POU1F1-and NR5A1-Cell Lineages
| Autor: | Gloria Silva-Román, Héctor Quezada, Baldomero Gonzales-Virla, Sandra Vela-Patiño, Laura Chávez-Macías, Ernesto Sosa, Aldo Ferreira-Hermosillo, Moisés Mercado, Blas López-Félix, Sophia Mercado-Medrez, Ana Laura Espinosa-de-los-Monteros, Claudia Ramírez-Rentería, Gerardo Guinto, Guadalupe Vargas-Ortega, Erick Gómez-Apo, Daniel Marrero-Rodríguez, Eduardo Peña-Martínez, Ana Laura Guzmán-Ortiz, Etual Espinosa-Cárdenas, Keiko Taniguchi-Ponciano |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adenoma
0301 basic medicine Spliceosome Proteome molecular markers lcsh:QH426-470 Biology Steroidogenic Factor 1 Article 03 medical and health sciences Exon alternative splicing 0302 clinical medicine Tandem Mass Spectrometry Biomarkers Tumor Genetics Humans Nanotechnology Protein Isoforms Cell Lineage Pituitary Neoplasms RNA Messenger RNA Neoplasm Gene mRNA isoforms Chromatography High Pressure Liquid Genetics (clinical) Oligonucleotide Array Sequence Analysis Principal Component Analysis Proteomic Profile Alternative splicing RNA Exons Hormones Neoplasm Proteins Cell biology lcsh:Genetics Gene Ontology 030104 developmental biology 030220 oncology & carcinogenesis RNA splicing Spliceosomes Gene chip analysis pituitary adenomas Transcription Factor Pit-1 Transcriptome Transcription Factors |
| Zdroj: | Genes, Vol 11, Iss 1422, p 1422 (2020) Genes Volume 11 Issue 12 |
| ISSN: | 2073-4425 |
| Popis: | Background: Pituitary adenomas (PA) are the second most common tumor in the central nervous system and have low counts of mutated genes. Splicing occurs in 95% of the coding RNA. There is scarce information about the spliceosome and mRNA-isoforms in PA, and therefore we carried out proteomic and transcriptomic analysis to identify spliceosome components and mRNA isoforms in PA. Methods: Proteomic profile analysis was carried out by nano-HPLC and mass spectrometry with a quadrupole time-of-flight mass spectrometer. The mRNA isoforms and transcriptomic profiles were carried out by microarray technology. With proteins and mRNA information we carried out Gene Ontology and exon level analysis to identify splicing-related events. Results: Approximately 2000 proteins were identified in pituitary tumors. Spliceosome proteins such as SRSF1, U2AF1 and RBM42 among others were found in PA. These results were validated at mRNA level, which showed up-regulation of spliceosome genes in PA. Spliceosome-related genes segregate and categorize PA tumor subtypes. The PA showed alterations in CDK18 and THY1 mRNA isoforms which could be tumor specific. Conclusions: Spliceosome components are significant constituents of the PA molecular machinery and could be used as molecular markers and therapeutic targets. Splicing-related genes and mRNA-isoforms profiles characterize tumor subtypes. |
| Databáze: | OpenAIRE |
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