Gene delivery to mammalian cells using a graphene nanoribbon platform
| Autor: | Hui-Chen Chang Foreman, Balaji Sitharaman, Gaurav Lalwani, Jaslin Kalra, Laurie T. Krug |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Biomedical Engineering 02 engineering and technology Carbon nanotube Gene delivery 010402 general chemistry 01 natural sciences Green fluorescent protein law.invention chemistry.chemical_compound law General Materials Science Fourier transform infrared spectroscopy chemistry.chemical_classification Sugar phosphates Graphene General Chemistry General Medicine Transfection 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Biochemistry Biophysics 0210 nano-technology DNA |
| Zdroj: | Journal of materials chemistry. B. 5(12) |
| ISSN: | 2050-7518 |
| Popis: | We have developed a novel oxidized graphene nanoribbon-based platform (O-GNR) for gene delivery of double-stranded DNA into mammalian cells. O-GNRs, synthesized via longitudinal unzipping of multi-walled carbon nanotubes (MWCNTs), exhibited efficient DNA loading of small dsDNA fragments. Fourier Transform Infrared Spectroscopy identified stretching peaks in the O-P-O and DNA sugar phosphate backbone that were consistent with DNA loading onto O-GNRs. The presence of salts in the loading buffer promoted DNA loading and effective dispersion of O-GNRs. DNA:O-GNR complexes were stable upon treatment with surfactants Tween 20 and Triton-X100. O-GNRs did not impact the viability of mammalian cells. Last, the detection of GFP expression upon transfection of the DNA:O-GNR complex indicated that the cargo DNA is expressed in the nucleus. Taken together, O-GNRs function as a platform for gene delivery to mammalian cells. |
| Databáze: | OpenAIRE |
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