Lutein transport by caco-2 TC-7 cells occurs partly by a facilitated process involving the scavenger receptor class B type I (SR-BI)
| Autor: | Lydia Abou, Henri Portugal, Emmanuelle Reboul, Marie-Josèphe Amiot, Patrick Borel, O. Ghiringhelli, C. Mikail, Dominique Jourdheuil-Rahmani, Denis Lairon, Marc André |
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| Přispěvatelé: | Institut Méditerranéen de Biochimie et Biologie de la Nutrition (IMRN), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
CD36 Antigens
Lutein endocrine system 030309 nutrition & dietetics Biochemistry Binding Competitive Intestinal absorption 03 medical and health sciences chemistry.chemical_compound medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Scavenger receptor Receptors Immunologic Molecular Biology Carotenoid ComputingMilieux_MISCELLANEOUS 030304 developmental biology chemistry.chemical_classification Receptors Scavenger 0303 health sciences Chromatography BETA-CAROTENE food and beverages Biological Transport Cell Biology Scavenger Receptors Class B Trypsin eye diseases ABSORPTION INTESTINALE Oleic acid Kinetics Enterocytes chemistry Intestinal Absorption Caco-2 Xanthophyll sense organs Caco-2 Cells Carrier Proteins medicine.drug Research Article |
| Zdroj: | Biochemical Journal Biochemical Journal, Portland Press, 2005, 387, pp.455-461 HAL |
| ISSN: | 0264-6021 1470-8728 |
| Popis: | The carotenoid lutein is thought to play a role in the human eye and to protect against age-related macular degeneration. Lutein transport in the human intestine has not been characterized. We examined lutein transport processes using Caco-2 TC-7 monolayers as a model for human intestinal epithelium. Purified lutein was mixed with phospholipids, lysophospholipids, cholesterol, mono-olein, oleic acid and taurocholate to obtain lutein-rich mixed micelles that mimicked those found under physiological conditions. The micelles were added to the apical side of Caco-2 TC-7 cell monolayers for 30 min or 3 h at 37 degrees C. Absorbed lutein, i.e. the sum of lutein recovered in the scraped cells and in the basolateral chamber, was quantified by HPLC. Transport rate was measured (i) as a function of time (from 15 to 60 min), (ii) as a function of micellar lutein concentration (from 1.5 to 15 microM), (iii) at 4 degrees C, (iv) in the basolateral to apical direction, (v) after trypsin pretreatment, (vi) in the presence of beta-carotene and/or lycopene, (vii) in the presence of increasing concentrations of antibody against SR-BI (scavenger receptor class B type 1) and (viii) in the presence of increasing concentrations of a chemical inhibitor of the selective transfer of lipids mediated by SR-BI, i.e. BLT1 (blocks lipid transport 1). The rate of transport of lutein as a function of time and as a function of concentration was saturable. It was significantly lower at 4 degrees C than at 37 degrees C (approx. 50%), in the basal to apical direction than in the opposite direction (approx. 85%), and after trypsin pretreatment (up to 45%). Co-incubation with beta-carotene, but not lycopene, decreased the lutein absorption rate (approx. 20%) significantly. Anti-SR-BI antibody and BLT1 significantly impaired the absorption rate (approx. 30% and 57% respectively). Overall, these results indicate that lutein absorption is, at least partly, protein-mediated and that some lutein is taken up through SR-BI. |
| Databáze: | OpenAIRE |
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