Genome-wide transcriptome profile of rice hybrids with and without Oryza rufipogon introgression reveals candidate genes for yield
| Autor: | Sarla Neelamraju, Surekha Agarwal, Shashi Rekha Thummala, Rajeshwari Ramanan, Haritha Guttikonda, Satendra K. Mangrauthia |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Candidate gene genetic structures Sequence analysis Quantitative Trait Loci lcsh:Medicine Introgression Biology Quantitative trait locus Genetic Introgression 01 natural sciences Article Plant breeding 03 medical and health sciences Gene Expression Regulation Plant Exome Sequencing Genetic variation Gene Regulatory Networks lcsh:Science Indel Plant Proteins Hybrid Genetics Multidisciplinary Gene Expression Profiling lcsh:R food and beverages Genetic Variation Oryza biology.organism_classification Oryza rufipogon Plant Leaves 030104 developmental biology lcsh:Q Edible Grain 010606 plant biology & botany |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) |
| ISSN: | 2045-2322 |
| Popis: | In this study, we compared genome-wide transcriptome profile of two rice hybrids, one with (test hybrid IR79156A/IL50-13) and the other without (control hybrid IR79156A/KMR3) O. rufipogon introgressions to identify candidate genes related to grain yield in the test hybrid. IL50-13 (Chinsurah Nona2 IET21943) the male parent (restorer) used in the test hybrid, is an elite BC4F8 introgression line of KMR3 with O. rufipogon introgressions. We identified 2798 differentially expressed genes (DEGs) in flag leaf and 3706 DEGs in panicle. Overall, 78 DEGs were within the major yield QTL qyld2.1 and 25 within minor QTL qyld8.2. The DEGs were significantly (p qyld2.1, Phenylalanine ammonia- lyase (PAL) (Os02t0626400-01, OsPAL2) showed 184 SNPs and 11 InDel variations and Similar to phenylalanine ammonia- lyase (Os02t0627100-01, OsPAL4) showed 205 SNPs and 13 InDel variations. Both PAL genes within qyld2.1 and derived from O. rufipogon are high priority candidate genes for increasing grain yield in rice. |
| Databáze: | OpenAIRE |
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