| Autor: |
Edgar Luís Catarí Ramones, Maria Teresa Salles Trevisan, Marília O. F. Goulart, Fabricia da Rocha Ferreira, Claudio Olea-Azar, Iara Barros Valentim, Fernanda Pérez-Cruz, Fabiane C. de Abreu |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
LWT - Food Science and Technology. 51:129-134 |
| ISSN: |
0023-6438 |
| Popis: |
The aim of this study was to characterize the complex between mangiferin (MGN) with β-cyclodextrin (β-CD) obtained through the co-evaporation method using Differential Scanning Calorimetry (DSC) and Fourier Transform Infrared spectroscopy (FT-IR), as well as to verify the antioxidant activity of this complex by means of radical scavenging activity toward 2,2′-diphenyl-1-picrylhydrazyl radical (RSA-DPPH ), with additional analysis of solvent effects, and Oxygen Radical Antioxidant Capacity-Fluorescein (ORAC-FL) assays. The protective effects of MGN and the MGN:β-CD complex against peroxyl radical-initiated membrane lipid peroxidation were also evaluated. The analysis of the solvent effects in the DPPH method demonstrated that at amounts of organic solvents lower than the ratio 50:50 (methanol–water or ethanol–water) there is a drawback, due to the hydrophobic nature of DPPH and should not be used. The antioxidant activity of the MGN:β-CD complex was higher than in its free form, as seen by both methods. The RSA-DPPH value was twofold larger for the complex while the ORAC-FL value was fifteenfold larger. Lipid peroxidation assays showed that the MGN:β-CD complex has a protective effect on the membrane that is as effective as the positive control (Trolox). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect