Characterization, isolation, and in vitro culture of leptomeningeal fibroblasts
| Autor: | Xinran Tong, Min Jun Li, Fadi Saadeh, Jean Wu, Adrienne Boire, Rajmohan Murali, Ján Remšík, Yudan Chi, Tejus Bale, Jenna Snyder, Camille Derderian, David Guber |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Amino Acid Transport System A Immunology Central nervous system Primary Cell Culture Cell Separation Biology Article Transcriptome Mice Meninges In vivo Parenchyma medicine Immunology and Allergy Animals Humans Cells Cultured Aged Mice Inbred BALB C Base Sequence Staining and Labeling Leptomeninges Fibroblasts Middle Aged Spinal cord Intercellular Adhesion Molecule-1 In vitro Mice Inbred C57BL medicine.anatomical_structure Neurology Child Preschool Female Neurology (clinical) Microdissection Biomarkers |
| Zdroj: | J Neuroimmunol |
| ISSN: | 1872-8421 |
| Popis: | Meninges, or the membranous coverings of the brain and spinal cord, play host to dozens of morbid pathologies. In this study we provide a method to isolate the leptomeningeal cell layer, identify leptomeninges in histologic slides, and maintain leptomeningeal fibroblasts in in vitro culture. Using an array of transcriptomic, histological, and cytometric analyses, we identified ICAM1 and SLC38A2 as two novel markers of leptomeningeal cells in vivo and in vitro. Our results confirm the fibroblastoid nature of leptomeningeal cells and their ability to form a sheet-like layer that covers the brain and spine parenchyma. These findings will enable researchers in central nervous system barriers to describe leptomeningeal cell functions in health and disease. |
| Databáze: | OpenAIRE |
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